Skip to main content
NCBI home page
Proceedings of the National Academy of Sciences of the United States of America logoLink to Proceedings of the National Academy of Sciences of the United States of America
. 1979 Feb;76(2):700–704. doi: 10.1073/pnas.76.2.700

Temperature-sensitive mutation in the initiation codon of the rIIB gene of bacteriophage T4.

D Belin, J Hedgpeth, G B Selzer, R H Epstein
PMCID: PMC383023  PMID: 284393

Abstract

We have determined the sequence of a ribosome-protected region of T4 rIIB mRNA labeled in vivo. The rIIB mutant HD263, which is temperature sensitive for translation, has an altered initiation codon (AUA instead of AUG). Because wild-type and HD263 proteins have the same fMet peptide, this AUA is used to initiate translation in the mutant rIIB mRNA. We have also identified a six-base sequence which is complementary to the 3" end of the 16S rRNA.

Full text

PDF
700

Images in this article

701Image
on p.701

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Belin D., Epstein R. H. A temperature-sensitive rIIB mutation which affects the synthesis of bacteriophage T4 rIIB protein. Virology. 1977 May 15;78(2):537–553. doi: 10.1016/0042-6822(77)90129-5. [DOI] [PubMed] [Google Scholar]
  2. Dube S. K., Marcker K. A., Clark B. F., Cory S. Nucleotide sequence of N-formyl-methionyl-transfer RNA. Nature. 1968 Apr 20;218(5138):232–233. doi: 10.1038/218232a0. [DOI] [PubMed] [Google Scholar]
  3. Dunn J. J., Buzash-Pollert E., Studier F. W. Mutations of bacteriophage T7 that affect initiation of synthesis of the gene 0.3 protein. Proc Natl Acad Sci U S A. 1978 Jun;75(6):2741–2745. doi: 10.1073/pnas.75.6.2741. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Fuller W., Hodgson A. Conformation of the anticodon loop intRNA. Nature. 1967 Aug 19;215(5103):817–821. doi: 10.1038/215817a0. [DOI] [PubMed] [Google Scholar]
  5. Gillespie D., Spiegelman S. A quantitative assay for DNA-RNA hybrids with DNA immobilized on a membrane. J Mol Biol. 1965 Jul;12(3):829–842. doi: 10.1016/s0022-2836(65)80331-x. [DOI] [PubMed] [Google Scholar]
  6. Guha A., Szybalski W., Salser W., Geiduschek E. P., Pulitzer J. F., Bolle A. Controls and polarity of transcription during bacteriophage T4 development. J Mol Biol. 1971 Jul 28;59(2):329–349. doi: 10.1016/0022-2836(71)90054-4. [DOI] [PubMed] [Google Scholar]
  7. Katz E. R., Brenner S. Genetic map of the beginning of the rIIB cistron of bacteriophage T4. Mol Gen Genet. 1975 Dec 30;143(1):101–104. doi: 10.1007/BF00269426. [DOI] [PubMed] [Google Scholar]
  8. Kramer R. A., Steitz J. A. Correction for non-uniform phosphate labeling of E. coli and phage RNAs synthesized in vivo. Biochem Biophys Res Commun. 1973 Oct 1;54(3):1198–1202. doi: 10.1016/0006-291x(73)90819-x. [DOI] [PubMed] [Google Scholar]
  9. Lee N., Carbon J. Nucleotide sequence of the 5' end of araBAD operon messenger RNA in Escherichia coli B/r. Proc Natl Acad Sci U S A. 1977 Jan;74(1):49–53. doi: 10.1073/pnas.74.1.49. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Neidhardt F. C., Bloch P. L., Smith D. F. Culture medium for enterobacteria. J Bacteriol. 1974 Sep;119(3):736–747. doi: 10.1128/jb.119.3.736-747.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Schmidt D. A., Mazaitis A. J., Kasai T., Bautz E. K. Involvement of a phage T4 sigma factor and an anti-terminator protein in the transcription of early T4 genes in vivo. Nature. 1970 Mar 14;225(5237):1012–1016. doi: 10.1038/2251012a0. [DOI] [PubMed] [Google Scholar]
  13. Selzer G., Bolle A., Krisch B., Epstein R. Construction and properties of recombinant plasmids containing the rII genes of bacteriophage T4. Mol Gen Genet. 1978 Feb 27;159(3):301–309. doi: 10.1007/BF00268267. [DOI] [PubMed] [Google Scholar]
  14. Shine J., Dalgarno L. The 3'-terminal sequence of Escherichia coli 16S ribosomal RNA: complementarity to nonsense triplets and ribosome binding sites. Proc Natl Acad Sci U S A. 1974 Apr;71(4):1342–1346. doi: 10.1073/pnas.71.4.1342. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Singer B. S., Gold L. A mutation that confers temperature sensitivity on the translation of rIIB in bacteriophage T4. J Mol Biol. 1976 May 25;103(3):627–646. doi: 10.1016/0022-2836(76)90221-7. [DOI] [PubMed] [Google Scholar]
  16. Smith G. R., Hedgpeth J. Oligo(A) not coded by DNA generating 3'-terminal heterogeneity in a lambda phage RNA. J Biol Chem. 1975 Jun 25;250(12):4818–4821. [PubMed] [Google Scholar]
  17. Steitz J. A., Bryan R. A. Two ribosome binding sites from the gene 0-3 messenger RNA of bacteriophages T7. J Mol Biol. 1977 Aug 25;114(4):527–543. doi: 10.1016/0022-2836(77)90176-0. [DOI] [PubMed] [Google Scholar]
  18. Steitz J. A., Jakes K. How ribosomes select initiator regions in mRNA: base pair formation between the 3' terminus of 16S rRNA and the mRNA during initiation of protein synthesis in Escherichia coli. Proc Natl Acad Sci U S A. 1975 Dec;72(12):4734–4738. doi: 10.1073/pnas.72.12.4734. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Steitz J. A. Polypeptide chain initiation: nucleotide sequences of the three ribosomal binding sites in bacteriophage R17 RNA. Nature. 1969 Dec 6;224(5223):957–964. doi: 10.1038/224957a0. [DOI] [PubMed] [Google Scholar]
  20. Uhlenbeck O. C., Baller J., Doty P. Complementary oligonucleotide binding to the anticodon loop of fMet-transfer RNA. Nature. 1970 Feb 7;225(5232):508–510. doi: 10.1038/225508a0. [DOI] [PubMed] [Google Scholar]
  21. Volckaert G., Jou W. M., Fiers W. Analysis of 32P-labeled bacteriophage MS2 RNA by a mini-fingerprinting procedure. Anal Biochem. 1976 May 7;72:433–446. doi: 10.1016/0003-2697(76)90551-0. [DOI] [PubMed] [Google Scholar]

Articles from Proceedings of the National Academy of Sciences of the United States of America are provided here courtesy of National Academy of Sciences

RESOURCES