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. 1979 Feb;76(2):760–764. doi: 10.1073/pnas.76.2.760

A general method for maximizing the expression of a cloned gene.

T M Roberts, R Kacich, M Ptashne
PMCID: PMC383043  PMID: 370836

Abstract

We present a method, utilizing a combination of restriction endonuclease cleavage and digestion with Escherichia coli exonuclease III and Aspergillus orizae nuclease S1, that allows us to position a restriction fragment bearing the promoter of the lacZ gene of E. coli at virtually any distance in front of any cloned gene. In particular, we have used this method to examine the effect on protein production of gene-promoter separation for the cro gene of phage lambda and to produce plasmids that, upon transformation into appropriate E. coli hosts, direct the synthesis of up to 190,000 cro protein monomers per cell.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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