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Proceedings of the National Academy of Sciences of the United States of America logoLink to Proceedings of the National Academy of Sciences of the United States of America
. 1979 Feb;76(2):806–808. doi: 10.1073/pnas.76.2.806

Change in nuclear potassium electrochemical activity and puffing of potassium-sensitive salivary chromosome regions during Chironomus development.

P Wuhrmann, H Ineichen, U Riesen-Willi, M Lezzi
PMCID: PMC383057  PMID: 284400

Abstract

Changes in nuclear K+ electrochemical activity and total nuclear K+ content in salivary glands of Chironomus tentans were measured with ion-selective microelectrodes based on valinomycin and with flameless atomic absorption spectrometry, respectively. The K+ activity increased by a factor of 2.6 and the total K+, by a factor of 1.5 as oligopausing larvae developed into prepupae. The extent of decondensation (puffing) of K+-sensitive regions in the polytene chromosomes underwent a parallel increase during this developmental event. In vitro culture of glands from oligopausing larvae resulted in similar changes with respect to nuclear K+ activity and puffing.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Barth L. J., Barth L. G. Effect of the potassium ion on induction of notochord from gastrula ectoderm of Rana pipiens. Biol Bull. 1974 Jun;146(3):313–325. doi: 10.2307/1540407. [DOI] [PubMed] [Google Scholar]
  2. Burkart T. Elektrolytveränderungen in Amphibiengeweben während der Larvenentwicklung, Metamorphose und Regneration (Xenopus laevis Daudin und Triturus cristatus carnifex. J Embryol Exp Morphol. 1972 Aug;28(1):57–76. [PubMed] [Google Scholar]
  3. CLEVER U. [Gene activity in the giant chromosomes of Chironomus tentans and its relation to development. I. Gene activation by ecdysone]. Chromosoma. 1961;12:607–675. doi: 10.1007/BF00328945. [DOI] [PubMed] [Google Scholar]
  4. Coles J. A., Tsacopoulos M. A method of making fine double-barrelled potassium-sensitive micro-electrodes for intracellular recording [proceedings]. J Physiol. 1977 Aug;270(1):12P–14P. [PubMed] [Google Scholar]
  5. KROEGER H. CHEMICAL NATURE OF THE SYSTEM CONTROLLING GENE ACTIVITIES IN INSECT CELLS. Nature. 1963 Dec 21;200:1234–1235. doi: 10.1038/2001234a0. [DOI] [PubMed] [Google Scholar]
  6. Kroeger H., Lezzi M. Regulation of gene action in insect development. Annu Rev Entomol. 1966;11:1–22. doi: 10.1146/annurev.en.11.010166.000245. [DOI] [PubMed] [Google Scholar]
  7. Kroeger H., Trösch W., Müller G. Changes in nuclear electrolytes of Chironomus thummi salivary gland cells during development. Exp Cell Res. 1973 Aug;80(2):329–339. doi: 10.1016/0014-4827(73)90304-2. [DOI] [PubMed] [Google Scholar]
  8. Lezzi M., Gilbert L. I. Control of gene activities in the polytene chromosomes of Chironomus tentans by ecdysone and juvenile hormone. Proc Natl Acad Sci U S A. 1969 Oct;64(2):498–503. doi: 10.1073/pnas.64.2.498. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Lezzi M., Gilbert L. I. Differential effects of K+ and Na+ on specific bands of isolated polytene chromosomes of Chironomus tentans. J Cell Sci. 1970 May;6(3):615–627. doi: 10.1242/jcs.6.3.615. [DOI] [PubMed] [Google Scholar]
  10. Lezzi M. Induktion eines Ecdyson-aktivierbaren Puff in isolierten Zellkernen von Chironomus durch KCL. Exp Cell Res. 1966 Oct;43(3):571–577. doi: 10.1016/0014-4827(66)90028-0. [DOI] [PubMed] [Google Scholar]
  11. Lezzi M. Spezifische Aktivitätssteigerung eines Balbianiringes durch Mg2+ in isolierten Zellkernen von Chironomus. Chromosoma. 1967;21(1):109–122. doi: 10.1007/BF00330549. [DOI] [PubMed] [Google Scholar]
  12. Masui Y., Meyerhof P. G., Miller M. A., Wasserman W. J. Roles of divalent cations in maturation and activation of vertebrate oocytes. Differentiation. 1977 Oct 20;9(1-2):49–57. doi: 10.1111/j.1432-0436.1977.tb01518.x. [DOI] [PubMed] [Google Scholar]
  13. Mazia D. The cell cycle. Sci Am. 1974 Jan;230(1):53–64. [PubMed] [Google Scholar]
  14. Meier P. C., Ammann D., Osswald H. F., Simon W. Review: ion-selective electrodes in clinical chemistry. Med Prog Technol. 1977 Jul 15;5(1):1–12. [PubMed] [Google Scholar]
  15. Moore R. D., Morrill G. A. A possible mechanism for concentrating sodium and potassium in the cell nucleus. Biophys J. 1976 May;16(5):527–533. doi: 10.1016/S0006-3495(76)85707-4. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Muir C., Whitley J. E. Variation in the nuclear sodium concentration of newt oocytes during maturation. J Cell Sci. 1972 Mar;10(2):335–338. doi: 10.1242/jcs.10.2.335. [DOI] [PubMed] [Google Scholar]
  17. Mähr R., Lezzi M., Eppenberger H. M. Mass isolation ofpolytene nuclei from Chironomus salivary glands. J Cell Sci. 1977;27:1–12. doi: 10.1242/jcs.27.1.1. [DOI] [PubMed] [Google Scholar]
  18. Okazaki K., Shull K. H., Farber E. Effects of ethionine on adenosine triphosphate levels and ionic composition of liver cell nuclei. J Biol Chem. 1968 Sep 25;243(18):4661–4666. [PubMed] [Google Scholar]
  19. Palmer L. G., Civan M. M. Distribution of Na+, K+ and Cl- between nucleus and cytoplasm in Chironomus salivary gland cells. J Membr Biol. 1977 May 6;33(1-2):41–61. doi: 10.1007/BF01869511. [DOI] [PubMed] [Google Scholar]
  20. Pieri C., Nagy I. Z., Nagy V. Z., Giuli C., Bertoni-Freddari C. Energy dispersive x-ray microanalysis of the electrolytes in biological bulk specimen. II. Age-dependent alterations in the monovalent ion contents of cell nucleus and cytoplasm in rat liver and brain cells. J Ultrastruct Res. 1977 Jun;59(3):320–331. doi: 10.1016/s0022-5320(77)90042-9. [DOI] [PubMed] [Google Scholar]
  21. Ringborg U., Rydlander L. Nucleolar-derived ribonucleic acid in chromosomes, nuclear sap, and cytoplasm of Chironomus tentans salivary gland cells. J Cell Biol. 1971 Nov;51(21):355–368. doi: 10.1083/jcb.51.2.355. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Robert M. Einfluss von Ionenstärke und pH auf die differentielle Dekondensation der Nukleoproteide isolierter speicheldrüsen-Zellkerne und -Chromosomen von Chironomus thummi. Chromosoma. 1971;36(1):1–33. doi: 10.1007/BF00326419. [DOI] [PubMed] [Google Scholar]
  23. Weissbach A. Eukaryotic DNA polymerases. Annu Rev Biochem. 1977;46:25–47. doi: 10.1146/annurev.bi.46.070177.000325. [DOI] [PubMed] [Google Scholar]

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