Abstract
Radioiodinated IgM isolated from a patient with Waldenström macroglobulinemia binds with high avidity (apparent Ka = 2.5 x 10(9) M-1) to freshly isolated human peripheral blood lymphocytes. Binding occurred through the Fc region of the molecule and involved a receptor specific for immunoglobulins of the IgM class. The avidity and specificity of this binding are consistent with a biologic role for IgM receptors on lymphocytes.
Full text
PDF![912](https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1055/383091/dbf4ae9c536b/pnas00002-0370.png)
![913](https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1055/383091/e81c2e51f19f/pnas00002-0371.png)
![914](https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1055/383091/a504b0c117a9/pnas00002-0372.png)
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Conradie J. D., Bubb M. O. Cmu4 domain of IgM has cytophilic activity for human lymphocytes. Nature. 1977 Jan 13;265(5590):160–161. doi: 10.1038/265160a0. [DOI] [PubMed] [Google Scholar]
- FRANKLIN E. C. Structural units of human 7S gamma globulin. J Clin Invest. 1960 Dec;39:1933–1941. doi: 10.1172/JCI104218. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Ferrarini M., Hoffman T., Fu S. M., Winchester R., Kunkel H. G. Receptors for IgM on certain human B lymphocytes. J Immunol. 1977 Oct;119(4):1525–1529. [PubMed] [Google Scholar]
- Ferrarini M., Moretta L., Mingari M. C., Tonda P., Pernis B. Human T cell receptor for IgM: specificity for the pentameric Fc fragment. Eur J Immunol. 1976 Jul;6(7):520–521. doi: 10.1002/eji.1830060714. [DOI] [PubMed] [Google Scholar]
- Gmelig-Meyling F., Van der Ham M., Ballieux R. E. Binding of IgM by human T lymphocytes. Scand J Immunol. 1976;5(5):487–495. doi: 10.1111/j.1365-3083.1976.tb00303.x. [DOI] [PubMed] [Google Scholar]
- Ishizaka T., Soto C. S., Ishizaka K. Mechanisms of passive sensitization. 3. Number of IgE molecules and their receptor sites on human basophil granulocytes. J Immunol. 1973 Aug;111(2):500–511. [PubMed] [Google Scholar]
- Lawrence D. A., Weigle W. O., Spiegelberg H. L. Immunoglobulins cytophilic for human lymphocytes, monocytes, and neutrophils. J Clin Invest. 1975 Feb;55(2):368–376. doi: 10.1172/JCI107940. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Marchalonis J. J. An enzymic method for the trace iodination of immunoglobulins and other proteins. Biochem J. 1969 Jun;113(2):299–305. doi: 10.1042/bj1130299. [DOI] [PMC free article] [PubMed] [Google Scholar]
- McConnell I., Hurd C. M. Lymphocyte receptors. II. Receptors for rabbit IgM on human T lymphocytes. Immunology. 1976 Jun;30(6):835–839. [PMC free article] [PubMed] [Google Scholar]
- Moretta L., Webb S. R., Grossi C. E., Lydyard P. M., Cooper M. D. Functional analysis of two human T-cell subpopulations: help and suppression of B-cell responses by T cells bearing receptors for IgM or IgG. J Exp Med. 1977 Jul 1;146(1):184–200. doi: 10.1084/jem.146.1.184. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Plaut A. G., Tomasi T. B., Jr Immunoglobulin M: pentameric Fcmu fragments released by trypsin at higher temperatures. Proc Natl Acad Sci U S A. 1970 Feb;65(2):318–322. doi: 10.1073/pnas.65.2.318. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Preud'homme J. L., Gonnot M., Tsapis A., Brouet J. C., Mihaesco C. Human T lymphocyte receptors for IgM: reactivity with monomeric 8S subunit. J Immunol. 1977 Dec;119(6):2206–2208. [PubMed] [Google Scholar]
- Rickles F. R., Hardin J. A., Pitlick F. A., Hoyer L. W., Conrad M. E. Tissue factor activity in lymphocyte cultures from normal individuals and patients with hemophilia A. J Clin Invest. 1973 Jun;52(6):1427–1434. doi: 10.1172/JCI107316. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Studier F. W. Analysis of bacteriophage T7 early RNAs and proteins on slab gels. J Mol Biol. 1973 Sep 15;79(2):237–248. doi: 10.1016/0022-2836(73)90003-x. [DOI] [PubMed] [Google Scholar]
- Unkeless J. C., Eisen H. N. Binding of monomeric immunoglobulins to Fc receptors of mouse macrophages. J Exp Med. 1975 Dec 1;142(6):1520–1533. doi: 10.1084/jem.142.6.1520. [DOI] [PMC free article] [PubMed] [Google Scholar]