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. 1979 Feb;76(2):912–914. doi: 10.1073/pnas.76.2.912

IgM receptors on human lymphocytes: detection by direct binding.

J A Hardin, K K Nakaoka, J M Carboni
PMCID: PMC383091  PMID: 284416

Abstract

Radioiodinated IgM isolated from a patient with Waldenström macroglobulinemia binds with high avidity (apparent Ka = 2.5 x 10(9) M-1) to freshly isolated human peripheral blood lymphocytes. Binding occurred through the Fc region of the molecule and involved a receptor specific for immunoglobulins of the IgM class. The avidity and specificity of this binding are consistent with a biologic role for IgM receptors on lymphocytes.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Conradie J. D., Bubb M. O. Cmu4 domain of IgM has cytophilic activity for human lymphocytes. Nature. 1977 Jan 13;265(5590):160–161. doi: 10.1038/265160a0. [DOI] [PubMed] [Google Scholar]
  2. FRANKLIN E. C. Structural units of human 7S gamma globulin. J Clin Invest. 1960 Dec;39:1933–1941. doi: 10.1172/JCI104218. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Ferrarini M., Hoffman T., Fu S. M., Winchester R., Kunkel H. G. Receptors for IgM on certain human B lymphocytes. J Immunol. 1977 Oct;119(4):1525–1529. [PubMed] [Google Scholar]
  4. Ferrarini M., Moretta L., Mingari M. C., Tonda P., Pernis B. Human T cell receptor for IgM: specificity for the pentameric Fc fragment. Eur J Immunol. 1976 Jul;6(7):520–521. doi: 10.1002/eji.1830060714. [DOI] [PubMed] [Google Scholar]
  5. Gmelig-Meyling F., Van der Ham M., Ballieux R. E. Binding of IgM by human T lymphocytes. Scand J Immunol. 1976;5(5):487–495. doi: 10.1111/j.1365-3083.1976.tb00303.x. [DOI] [PubMed] [Google Scholar]
  6. Ishizaka T., Soto C. S., Ishizaka K. Mechanisms of passive sensitization. 3. Number of IgE molecules and their receptor sites on human basophil granulocytes. J Immunol. 1973 Aug;111(2):500–511. [PubMed] [Google Scholar]
  7. Lawrence D. A., Weigle W. O., Spiegelberg H. L. Immunoglobulins cytophilic for human lymphocytes, monocytes, and neutrophils. J Clin Invest. 1975 Feb;55(2):368–376. doi: 10.1172/JCI107940. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Marchalonis J. J. An enzymic method for the trace iodination of immunoglobulins and other proteins. Biochem J. 1969 Jun;113(2):299–305. doi: 10.1042/bj1130299. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. McConnell I., Hurd C. M. Lymphocyte receptors. II. Receptors for rabbit IgM on human T lymphocytes. Immunology. 1976 Jun;30(6):835–839. [PMC free article] [PubMed] [Google Scholar]
  10. Moretta L., Webb S. R., Grossi C. E., Lydyard P. M., Cooper M. D. Functional analysis of two human T-cell subpopulations: help and suppression of B-cell responses by T cells bearing receptors for IgM or IgG. J Exp Med. 1977 Jul 1;146(1):184–200. doi: 10.1084/jem.146.1.184. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Plaut A. G., Tomasi T. B., Jr Immunoglobulin M: pentameric Fcmu fragments released by trypsin at higher temperatures. Proc Natl Acad Sci U S A. 1970 Feb;65(2):318–322. doi: 10.1073/pnas.65.2.318. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Preud'homme J. L., Gonnot M., Tsapis A., Brouet J. C., Mihaesco C. Human T lymphocyte receptors for IgM: reactivity with monomeric 8S subunit. J Immunol. 1977 Dec;119(6):2206–2208. [PubMed] [Google Scholar]
  13. Rickles F. R., Hardin J. A., Pitlick F. A., Hoyer L. W., Conrad M. E. Tissue factor activity in lymphocyte cultures from normal individuals and patients with hemophilia A. J Clin Invest. 1973 Jun;52(6):1427–1434. doi: 10.1172/JCI107316. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Studier F. W. Analysis of bacteriophage T7 early RNAs and proteins on slab gels. J Mol Biol. 1973 Sep 15;79(2):237–248. doi: 10.1016/0022-2836(73)90003-x. [DOI] [PubMed] [Google Scholar]
  15. Unkeless J. C., Eisen H. N. Binding of monomeric immunoglobulins to Fc receptors of mouse macrophages. J Exp Med. 1975 Dec 1;142(6):1520–1533. doi: 10.1084/jem.142.6.1520. [DOI] [PMC free article] [PubMed] [Google Scholar]

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