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. 1979 Mar;76(3):1150–1154. doi: 10.1073/pnas.76.3.1150

Nucleotide sequence of the region of an origin of replication of the antibiotic resistance plasmid R6K.

D M Stalker, R Kolter, D R Helinski
PMCID: PMC383207  PMID: 375227

Abstract

A 2.1-kilobase segment of the antibiotic resistance plasmid R6K carries sufficient information to replicate as a plasmid in Escherichia coli. This segment contains a functional origin of replication and a structural gene for a protein, designated pi, that is required for the initiation of R6K replication. The nucleotide sequence of a 520-base-pair portion of this 2.1-kilobase segment that includes the functional origin of replication and the region adjacent to the start of the pi structural gene was determined. A striking feature of the sequence is the presence of seven 22-base-pair direct repeats joined in tandem in the region adjacent to the start of the pi gene. A possible role of the tandem repeats in the regulation of expression of the pi protein and the control of initiation of replication of the plasmid R6K is discussed.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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