Skip to main content
PMC home page
Proceedings of the National Academy of Sciences of the United States of America logoLink to Proceedings of the National Academy of Sciences of the United States of America
. 1979 Mar;76(3):1284–1287. doi: 10.1073/pnas.76.3.1284

Vasopressin stimulation of mouse 3T3 cell growth.

E Rozengurt, A Legg, P Pettican
PMCID: PMC383235  PMID: 312501

Abstract

Vasopressin is shown to be a potent mitogen for Swiss 3T3 cells. The hormone (1--10 ng/ml) causes a striking shift of the dose--response curve for the effect of serum on thymidine incorporation by cultures of 3T3 cells arrested in the G1/G0 phase of the cell cycle. In the absence of added serum, the effect of vasopressin on DNA synthesis is greatly potentiated by insulin, epidermal growth factor, and a factor isolated from medium conditioned by simian virus 40-infected baby hamster kidney cells. The mitogenic effect of vasopressin is dependent on time and hormone concentration. In the presence of insulin, the half-maximal effect elicited by the peptide is obtained at 0.6 ng/ml. [Arg]Vasopressin and [Lys]vasopressin are equally potent. The vasopressins are 10(3)-fold more potent than oxytocin. In the presence of a low (2.5%) concentration of serum, vasopressins stimulate cell proliferation.

Full text

PDF
1284

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bourne H. R., Rozengurt E. An 18,000 molecular weight polypeptide induces early events and stimulates DNA synthesis in cultured cells. Proc Natl Acad Sci U S A. 1976 Dec;73(12):4555–4559. doi: 10.1073/pnas.73.12.4555. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Chen J. L., Babcock D. F., Lardy H. A. Norepinephrine, vasopressin, glucagon, and A23187 induce efflux of calcium from an exchangeable pool in isolated rat hepatocytes. Proc Natl Acad Sci U S A. 1978 May;75(5):2234–2238. doi: 10.1073/pnas.75.5.2234. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Dousa T. P. Cyclic nucleotides in the cellular action of neurohypophyseal hormones. Fed Proc. 1977 May;36(6):1867–1871. [PubMed] [Google Scholar]
  4. Gospodarowicz D., Moran J. S. Growth factors in mammalian cell culture. Annu Rev Biochem. 1976;45:531–558. doi: 10.1146/annurev.bi.45.070176.002531. [DOI] [PubMed] [Google Scholar]
  5. Hechter O., Terada S., Spitsberg V., Nakahara T., Nakagawaga S. H., Flouret G. Neurohypophyseal hormone-responsive renal adenylate cyclase. III. Relationship between affinity and intrinsic activity in neurohypophyseal hormones and structural analogs. J Biol Chem. 1978 May 10;253(9):3230–3237. [PubMed] [Google Scholar]
  6. Holley R. W. Control of growth of mammalian cells in cell culture. Nature. 1975 Dec 11;258(5535):487–490. doi: 10.1038/258487a0. [DOI] [PubMed] [Google Scholar]
  7. Hunt N. H., Perris A. D., Sandford P. A. Role of vasopressin in the mitotic response of rat bone marrow cells to haemorrhage. J Endocrinol. 1977 Jan;72(1):5–16. doi: 10.1677/joe.0.0720005. [DOI] [PubMed] [Google Scholar]
  8. Jard S., Roy C., Barth T., Rajerison R., Bockaert J. Antidiuretic hormone-sensitive kidney adenylate cyclase. Adv Cyclic Nucleotide Res. 1975;5:31–52. [PubMed] [Google Scholar]
  9. Mierzejewski K., Rozengurt E. Vitamin B12 enhances the stimulation of DNA synthesis by serum in resting cultures of 3T6 cells. Exp Cell Res. 1977 May;106(2):394–397. doi: 10.1016/0014-4827(77)90187-2. [DOI] [PubMed] [Google Scholar]
  10. Miller R. P., Husain F., Svensson M., Lohin S. Enhancement of [3H-methyl]thymidine incorporation and replication of rat chondrocytes grown in tissue culture by plasma, tissue extracts and vasopressin. Endocrinology. 1977 May;100(5):1365–1375. doi: 10.1210/endo-100-5-1365. [DOI] [PubMed] [Google Scholar]
  11. Moore G., Lutterodt A., Burford G., Lederis K. A highly specific antiserum for arginine vasopressin. Endocrinology. 1977 Nov;101(5):1421–1435. doi: 10.1210/endo-101-5-1421. [DOI] [PubMed] [Google Scholar]
  12. Pettengill O. S., Faulkner C. S., Wurster-Hill D. H., Maurer L. H., Sorenson G. D., Robinson A. G., Zimmerman E. A. Isolation and characterization of a hormone-producing cell line from human small cell anaplastic carcinoma of the lung. J Natl Cancer Inst. 1977 Mar;58(3):511–518. doi: 10.1093/jnci/58.3.511. [DOI] [PubMed] [Google Scholar]
  13. Primack A. The production of markers by bronchogenic carcinoma: a review. Semin Oncol. 1974 Sep;1(3):235–244. [PubMed] [Google Scholar]
  14. Rozengurt E., Heppel L. A. Serum rapidly stimulates ouabain-sensitive 86-RB+ influx in quiescent 3T3 cells. Proc Natl Acad Sci U S A. 1975 Nov;72(11):4492–4495. doi: 10.1073/pnas.72.11.4492. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Smith G. L. Increased ouabain-sensitive 86Rubidium uptake after mitogenic stimulation of quiescent chicken embryo fibroblasts with purified multiplication-stimulating activity. J Cell Biol. 1977 Jun;73(3):761–767. doi: 10.1083/jcb.73.3.761. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Smith J. B., Rozengurt E. Lithium transport by fibroblastic mouse cells: characterization and stimulation by serum and growth factors in quiescent cultures. J Cell Physiol. 1978 Dec;97(3 Pt 2 Suppl 1):441–449. doi: 10.1002/jcp.1040970319. [DOI] [PubMed] [Google Scholar]
  17. Smith J. B., Rozengurt E. Serum stimulates the Na+,K+ pump in quiescent fibroblasts by increasing Na+ entry. Proc Natl Acad Sci U S A. 1978 Nov;75(11):5560–5564. doi: 10.1073/pnas.75.11.5560. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. TODARO G. J., GREEN H. Quantitative studies of the growth of mouse embryo cells in culture and their development into established lines. J Cell Biol. 1963 May;17:299–313. doi: 10.1083/jcb.17.2.299. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Tupper J. T., Zorgniotti F., Mills B. Potassium transport and content during G1 and S phase following serum stimulation of 3T3 cells. J Cell Physiol. 1977 Jun;91(3):429–440. doi: 10.1002/jcp.1040910313. [DOI] [PubMed] [Google Scholar]

Articles from Proceedings of the National Academy of Sciences of the United States of America are provided here courtesy of National Academy of Sciences

RESOURCES