Skip to main content
PMC home page
Proceedings of the National Academy of Sciences of the United States of America logoLink to Proceedings of the National Academy of Sciences of the United States of America
. 1979 Mar;76(3):1464–1468. doi: 10.1073/pnas.76.3.1464

In vitro transformation of mouse bone marrow cells by the polycythemic strain of Friend leukemia virus.

R Revoltella, L Bertolini, C Friend
PMCID: PMC383273  PMID: 86991

Abstract

Strains of Friend leukemia virus (FLV) that are associated with polycythemia contain the defective spleen focus-forming virus (SFFV). To determine whether the transforming ability of FLV was affected by the presence of this second agent, DBA/2J mouse bone marrow cells were infected in vitro. Criteria for transformation were the establishment of permanent lines, growth on semisolid agarose, and the production of tumors at the site of inoculation in syngeneic hosts. Two lines of immature hematopoietic cells that grow in suspension originated from the infected cultures. Each has an almost diploid karyotype (38-39 chromosomes) and 3-4 metacentric chromosomes. These transformed cells express gp71 viral envelope glycoprotein and p30 viral core protein antigens. Virus production was measured by reverse transcriptase (RNA-dependent DNA polymerase) activity of the virions released into the medium. The virus, assayed in vivo for infectivity, has SFFV activity but is attenuated for leukemogenicity. The stimulation of hemoglobin synthesis in the cells grown in medium supplemented with dimethyl sulfoxide or hexamethylene bisacetamide indicates that the cells are erythroid in origin. SFFV may have a function analogous to erythropoietin in influencing the process of transformation by FLV.

Full text

PDF
1464

Images in this article

1466Image
on p.1466
1466Image
on p.1466
1467Image
on p.1467

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. AXELRAD A. A., STEEVES R. A. ASSAY FOR FRIEND LEUKEMIA VIRUS: RAPID QUANTITATIVE METHOD BASED ON ENUMERATION OF MACROSCOPIC SPLEEN FOCI IN MICE. Virology. 1964 Nov;24:513–518. doi: 10.1016/0042-6822(64)90199-0. [DOI] [PubMed] [Google Scholar]
  2. Bilello J. A., Strand M., August J. T. Murine sarcoma virus gene expression: transformants which express viral envelope glycoprotein in the absence of the major internal protein and infectious particles. Proc Natl Acad Sci U S A. 1974 Aug;71(8):3234–3238. doi: 10.1073/pnas.71.8.3234. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Brown W. M., Axelrad A. A. Effect of Friend leukemia virus on megakaryocytes and platelets in mice. Int J Cancer. 1976 Dec 15;18(6):764–773. doi: 10.1002/ijc.2910180607. [DOI] [PubMed] [Google Scholar]
  4. Clarke B. J., Axelrad A. A., Shreeve M. M., McLeod D. L. Erythroid colony induction without erythropoietin by Friend leukemia virus in vitro. Proc Natl Acad Sci U S A. 1975 Sep;72(9):3556–3560. doi: 10.1073/pnas.72.9.3556. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Cloyd M. W., Bolognesi D. P., Bigner D. D. Immunofluorescent analysis of expression of the RNA tumor virus major glycoprotein, gp71, on the surfaces of normal murine cells. Cancer Res. 1977 Mar;37(3):931–938. [PubMed] [Google Scholar]
  6. Dexter T. M., Scott D., Teich N. M. Infection of bone marrow cells in vitro with FLV: effects on stem cell proliferation, differentiation and leukemogenic capacity. Cell. 1977 Oct;12(2):355–364. doi: 10.1016/0092-8674(77)90111-8. [DOI] [PubMed] [Google Scholar]
  7. FRIEND C. Cell-free transmission in adult Swiss mice of a disease having the character of a leukemia. J Exp Med. 1957 Apr 1;105(4):307–318. doi: 10.1084/jem.105.4.307. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Fibach E., Reuben R. C., Rifkind R. A., Marks P. A. Effect of hexamethylene bisacetamide on the commitment to differentiation of murine erythroleukemia cells. Cancer Res. 1977 Feb;37(2):440–444. [PubMed] [Google Scholar]
  9. Flickinger J. T., Gentile J. M. Influence of sensitization with bacterial antigens on the replication of Friend spleen focus-forming virus. Can J Microbiol. 1971 Apr;17(4):481–486. doi: 10.1139/m71-080. [DOI] [PubMed] [Google Scholar]
  10. Friend C., Marovitz W., Henie G., Henie W., Tsuei D., Hirschhorn K., Holland J. G., Cuttner J. Observations on cell lines derived from a patient with Hodgkin's disease. Cancer Res. 1978 Aug;38(8):2581–2591. [PubMed] [Google Scholar]
  11. Friend C., Patuleia M. C., De Harven E. Erythrocytic maturation in vitro of murine (Friend) virus-induced leukemic cells. Natl Cancer Inst Monogr. 1966 Sep;22:505–522. [PubMed] [Google Scholar]
  12. Friend C., Scher W., Holland J. G., Sato T. Hemoglobin synthesis in murine virus-induced leukemic cells in vitro: stimulation of erythroid differentiation by dimethyl sulfoxide. Proc Natl Acad Sci U S A. 1971 Feb;68(2):378–382. doi: 10.1073/pnas.68.2.378. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Gabelman N., Scher W., Friend C. Alterations in macromolecular synthesis and cellular growth in mouse embryo fibroblasts infected with Friend leukemia virus. Int J Cancer. 1974 Mar 15;13(3):343–352. doi: 10.1002/ijc.2910130310. [DOI] [PubMed] [Google Scholar]
  14. Golde D. W., Bersch N., Friend C., Tsuei D., Marovitz W. Transformation of DBA/2 mouse fetal liver cells infected in vitro by the anemic strain of Friend leukemia virus. Proc Natl Acad Sci U S A. 1979 Feb;76(2):962–966. doi: 10.1073/pnas.76.2.962. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Golde D. W., Faille A., Sullivan A., Friend C. Granulocytic stem cells in Friend leukemia. Cancer Res. 1976 Jan;36(1):115–119. [PubMed] [Google Scholar]
  16. METCALF D., FURTH J., BUFFETT R. F. Pathogenesis of mouse leukemia caused by Friend virus. Cancer Res. 1959 Jan;19(1):52–58. [PubMed] [Google Scholar]
  17. MIRAND E. A., PRENTICE T., HOFFMAN J. G., GRACE J. T., Jr Effect of Friend virus in Swiss and DBA/1 mice on Fe59 uptake. Proc Soc Exp Biol Med. 1961 Feb;106:423–426. doi: 10.3181/00379727-106-26358. [DOI] [PubMed] [Google Scholar]
  18. Mirand E. A. Erythropoietic response of animals infected with various strains of Friend virus. Natl Cancer Inst Monogr. 1966 Sep;22:483–503. [PubMed] [Google Scholar]
  19. Okunewick J. P. The role of committed and uncommitted hematopoietic stem cells as targets for Rauscher and Friend leukemia virus. Biomedicine. 1977 May;26(3):152–157. [PubMed] [Google Scholar]
  20. Orkin S. H., Harosi F. I., Leder P. Differentiation in erythroleukemic cells and their somatic hybrids. Proc Natl Acad Sci U S A. 1975 Jan;72(1):98–102. doi: 10.1073/pnas.72.1.98. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Ostertag W., Melderis H., Steinheider G., Kluge N., Dube S. Synthesis of mouse haemoglobin and globin mRNA in leukaemic cell cultures. Nat New Biol. 1972 Oct 25;239(95):231–234. doi: 10.1038/newbio239231a0. [DOI] [PubMed] [Google Scholar]
  22. Rosenberg N., Baltimore D. A quantitative assay for transformation of bone marrow cells by Abelson murine leukemia virus. J Exp Med. 1976 Jun 1;143(6):1453–1463. doi: 10.1084/jem.143.6.1453. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Scher W., Friend C. Breakage of DNA and alterations in folded genomes by inducers of differentiation in Friend erythroleukemic cells. Cancer Res. 1978 Mar;38(3):841–849. [PubMed] [Google Scholar]
  24. Steeves R. A., Fjelde A., Mirand E. A. Helper activity of human leukemic tissue extracts for leukemia virus expression in mice. Proc Natl Acad Sci U S A. 1971 Oct;68(10):2391–2395. doi: 10.1073/pnas.68.10.2391. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Steeves R. A., Mirand E. A., Thomson S., Avila L. Enhancement of spleen focus formation and virus replication in Friend virus-infected mice. Cancer Res. 1969 May;29(5):1111–1116. [PubMed] [Google Scholar]
  26. Tambourin P. E., Wendling F. Target cell for oncogenic action of polycythaemia-inducing Friend virus. Nature. 1975 Jul 24;256(5515):320–322. doi: 10.1038/256320a0. [DOI] [PubMed] [Google Scholar]

Articles from Proceedings of the National Academy of Sciences of the United States of America are provided here courtesy of National Academy of Sciences

RESOURCES