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. 1979 Apr;76(4):1585–1588. doi: 10.1073/pnas.76.4.1585

Isolation of maxicircle component of kinetoplast DNA from hemoflagellate protozoa.

L Simpson
PMCID: PMC383434  PMID: 286999

Abstract

Maxicircle DNA has been isolated from the kinetoplast DNA of Leishmania tarentolae culture forms by bouyant separation in CsCl in the presence of the A + T-binding dye Hoechst 33258, after liberation from the kinetoplast DNA network by cleavage with the single-hit restriction endonuclease EcoRI. The purified linearized maxicircle DNA has a density in CsCl of 1.681 g/cm3 (79% A + T) and a molecular weight of approximately 18-20 x 10(6). The maxicircle molecule exhibited intramolecular base composition heterogeneity ranging from 85% A + T to 74% A + T.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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