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. 1979 Apr;76(4):1858–1862. doi: 10.1073/pnas.76.4.1858

Secretion and cell-surface growth are blocked in a temperature-sensitive mutant of Saccharomyces cerevisiae.

P Novick, R Schekman
PMCID: PMC383491  PMID: 377286

Abstract

Saccharomyces cerevisiae cells contain a small internal pool of the secretory enzymes invertase and acid phosphatase. This pool increases up to 8-fold at 37 degrees C in a temperature-sensitive, secretion-defective mutant strain (sec 1-1). Cell division and incorporation of a sulfate permease activity stop abruptly at the restrictive temperature, while protein synthesis continues for several hours. Electron microscopy of mutant cells incubated at 37 degrees C reveals a large increase in the number of intracellular membrane-bound vesicles, which are shown by histochemical staining to contain the accumulated acid phosphatase. The vesicles are removed and the accumulated enzymes are secreted when cells are returned to a permissive temperature in the presence or absence of cycloheximide. These results are consistent with a vesicle intermediate in the yeast secretory pathway and suggest that exocytosis may contribute to cell-surface growth.

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These references are in PubMed. This may not be the complete list of references from this article.

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