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. 1979 Apr;76(4):1952–1956. doi: 10.1073/pnas.76.4.1952

Mutation of the non-Mendelian suppressor, Ψ+, in yeast by hypertonic media

Arjun Singh 1,*, Cynthia Helms 1, Fred Sherman 1
PMCID: PMC383511  PMID: 16592643

Abstract

The Ψ+ extrachromosomal determinant in the yeast Saccharomyces cerevisiae suppresses certain UAA markers and increases the efficiency of suppression of UAA suppressors and certain frameshift suppressors. Although the exact nature of Ψ+ determinant is unknown, it is believed to be a self-replicating cytoplasmic factor affecting some component of the translational machinery. In this report we describe growth conditions for efficient mutation or elimination of the Ψ+ determinant. Incubation of Ψ+ cultures in hypertonic nutrient medium resulted in rapid conversion to a culture containing predominantly Ψ- cells during the growth cycle. The kinetics of Ψ+ to Ψ- conversion established that the occurrence of Ψ- cells was due to induction and not to selection of pre-existing Ψ- cells. The results suggest that the replication of the Ψ+ determinant is sensitive to hypertonic conditions.

Keywords: nonsense suppression, Saccharomyces cerevisiae, cytoplasmic inheritance

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1952

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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