Figure 4.

Detection of DENV replication in neurons. Cells in mixed primary cell cultures from human CNS treated with DENV-2 were examined after 5 days by indirect immunofluorescence in an epifluorescence microscope. The monoclonal antibody raised against NS3 DENV allowed the assessing of the active viral replication in the brain primary cell cultures, (b) and (d) while the antibody cell markers enable the identification of the cell type as neurons. (c) and (d) Original magnification was 600x, but the pictures were cropped to improve presentation. Arrows point to virus replication sites in human neurons.