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. 1979 May;76(5):2163–2164. doi: 10.1073/pnas.76.5.2163

Further studies on the mode of action of the heme-controlled translational inhibitor: stimulating protein acts at level of binary complex formation.

C de Haro, S Ochoa
PMCID: PMC383557  PMID: 287053

Abstract

Previous work has shown that (i) at physiological concentrations of eukaryotic initiation factor 2 (eIF-2), formation of the ternary complex eIF-2-GTP-Met-tRNAi, which precedes the assembly of a 40S initiation complex, requires the presence of eIF-2 stimulating protein (ESP) and (ii) the interaction of eIF-2 with ESP is blocked by the translational inhibitor which, in reticulocyte lysates, is activated in the absence of hemin. Present evidence indicates that formation of the ternary complex is preceded by formation of the binary complex eIF-2-GTP and that ESP acts at the level of binary complex formation.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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