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. Author manuscript; available in PMC: 2014 Nov 1.
Published in final edited form as: Cytokine. 2013 Sep 8;64(2):10.1016/j.cyto.2013.08.005. doi: 10.1016/j.cyto.2013.08.005

Figure 5.

Figure 5

NE and ATP synergistically induce IL-6 release by neonatal pHDMECs primarily through 2-ARs. Supernatants were collected after 8 hrs and analyzed by ELISA. (A) Cells treated with 50 µM or 100 µM ATP alone significantly increased IL-6 release versus DM(p<0.01). Cells treated with 50 µM ATP plus NE (10−5 M or 10−6 M) or 100 µM ATP plus NE (10−5 M or 10−6 M) synergistically induced IL-6 release. ***p<0.001, **p<0.01, *p<0.05 versus additive effect of each agent alone. (B) Cells treated with 100 µM ATP and 10−6 M NE resulted in synergistic release of IL- 6 (p<0.001). Pretreatment with 10−5 M or 10−6 M Prop or 10−5 M ICI abolished this effect (***p<0.001) while pretreatment with phentolamine had no effect. 10−6 M Prop + 10−6 M NE is statistically different from 10−6 M NE alone (***p<0.001) but not from DM and 10−6 M Prop + 50 µM ATP is not different from 50 µM ATP. 10−5 M ICI +10−6 M NE differs from 10−5 M NE (***p<0.001) but not from DM. Each graph represents the average of two experiments. Error bars represent the mean +/-SD.