Table 1.
RA | Neurite outgrowth |
||||||
---|---|---|---|---|---|---|---|
d2 | d3 | d5 | d6 | d8 | d10 | d12 | |
Empty vector | – | – | – | + | + | + | + |
Tbx6 overexpression | – | – | – | – | + | + | + |
scrambled shRNA | – | – | – | + | + | + | + |
Tbx6 shRNA | – | + | + | + | + | + | + |
P19CL6-MLC-2v-GFP cells stably transfected with empty vector, Tbx6 expression construct, scrambled shRNA, or Tbx6 shRNA were treated with RA for 4 days (d), and neurite outgrowth was assessed on the days indicated by light microscopy.