Abstract
A restriction map of hepatitis B virion DNA was established after cloning of the whole viral genome in Escherichia coli. By use of EcoRI, Xho I, Bgl II, Xba I, BamHI, HincII, and Hae III endonucleases, a total of 28 restriction sites were mapped. The single-stranded region was localized on the restriction map and 5' end of the short strand was mapped at a fixed position.
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- Adams J. M., Jeppesen P. G., Sanger F., Barrell B. G. Nucleotide sequence from the coat protein cistron of R17 bacteriophage RNA. Nature. 1969 Sep 6;223(5210):1009–1014. doi: 10.1038/2231009a0. [DOI] [PubMed] [Google Scholar]
- Blumberg B. S. Australia antigen and the biology of hepatitis B. Science. 1977 Jul 1;197(4298):17–25. doi: 10.1126/science.325649. [DOI] [PubMed] [Google Scholar]
- Charnay P., Louise A., Fritsch A., Perrin D., Tiollais P. Bacteriophage lambda-E. coli K12 vector-host system for gene cloning and expression under lactose promoter control. II. DNA fragment insertion at the vicinity of the lac UV5 promoter. Mol Gen Genet. 1979 Feb 26;170(2):171–178. doi: 10.1007/BF00337793. [DOI] [PubMed] [Google Scholar]
- Dane D. S., Cameron C. H., Briggs M. Virus-like particles in serum of patients with Australia-antigen-associated hepatitis. Lancet. 1970 Apr 4;1(7649):695–698. doi: 10.1016/s0140-6736(70)90926-8. [DOI] [PubMed] [Google Scholar]
- Fritsch A., Pourcel C., Charnay P., Tiollais P. Clonage du génome du virus de l'hépatite B dans Escherichia coli. C R Acad Sci Hebd Seances Acad Sci D. 1978 Dec;287(16):1453–1456. [PubMed] [Google Scholar]
- Hruska J. F., Clayton D. A., Rubenstein J. L., Robinson W. S. Structure of hepatitis B Dane particle DNA before and after the Dane particle DNA polymerase reaction. J Virol. 1977 Feb;21(2):666–672. doi: 10.1128/jvi.21.2.666-672.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Landers T. A., Greenberg H. B., Robinson W. S. Structure of hepatitis B Dane particle DNA and nature of the endogenous DNA polymerase reaction. J Virol. 1977 Aug;23(2):368–376. doi: 10.1128/jvi.23.2.368-376.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Maniatis T., Jeffrey A., van deSande H. Chain length determination of small double- and single-stranded DNA molecules by polyacrylamide gel electrophoresis. Biochemistry. 1975 Aug 26;14(17):3787–3794. doi: 10.1021/bi00688a010. [DOI] [PubMed] [Google Scholar]
- Maupas P., Coursaget P., Goudeau A., Drucker J., Sankale M., Linhard J., Diebolt G. Virus de l'hépatite B et cancer primitif du foie: relation entre hépatite B, cirrhose et cancer primitif du foie. Ann Microbiol (Paris) 1977 Feb-Mar;128(2):245–253. [PubMed] [Google Scholar]
- Maxam A. M., Gilbert W. A new method for sequencing DNA. Proc Natl Acad Sci U S A. 1977 Feb;74(2):560–564. doi: 10.1073/pnas.74.2.560. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Robinson W. S. The genome of hepatitis B virus. Annu Rev Microbiol. 1977;31:357–377. doi: 10.1146/annurev.mi.31.100177.002041. [DOI] [PubMed] [Google Scholar]
- Sanger F., Coulson A. R. A rapid method for determining sequences in DNA by primed synthesis with DNA polymerase. J Mol Biol. 1975 May 25;94(3):441–448. doi: 10.1016/0022-2836(75)90213-2. [DOI] [PubMed] [Google Scholar]
- Summers J., O'Connell A., Maupas P., Goudeau A., Coursaget P., Drucker J. Hepatitis B virus DNA in primary hepatocellular carcinoma tissue. J Med Virol. 1978;2(3):207–214. doi: 10.1002/jmv.1890020304. [DOI] [PubMed] [Google Scholar]
- Summers J., O'Connell A., Millman I. Genome of hepatitis B virus: restriction enzyme cleavage and structure of DNA extracted from Dane particles. Proc Natl Acad Sci U S A. 1975 Nov;72(11):4597–4601. doi: 10.1073/pnas.72.11.4597. [DOI] [PMC free article] [PubMed] [Google Scholar]