pHg/pSILBAγ vector system for efficient gene silencing in homobasidiomycetes: optimization of ihpRNA – triggering in the mycorrhizal fungus Laccaria bicolor

In the article ‘pHg/pSILBAγ vector system for efficient gene silencing in homobasidiomycetes: optimization of ihpRNA – triggering in the mycorrhizal fungus Laccaria bicolor’ (Kemppainen and Pardo, 2009), with reference to fig. 2, we would like to clarify the following points:

In plasmid pHg, the NotI site is not unique, hence this restriction enzyme cannot be used to linearize this vector. Plasmid pHg can be linearized with SacI or XbaI whose restriction sites are unique.

In pSILBA, NotI and SacI sites are unique thus the plasmid can be linearized with these restriction enzymes under the condition that the hpDNA sequence is free of these sites.

We apologize for this mistake.