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. 1979 Jun;76(6):2735–2739. doi: 10.1073/pnas.76.6.2735

Chromosomal assignment of the mouse kappa light chain genes.

D Swan, P D'Eustachio, L Leinwand, J Seidman, D Keithley, F H Ruddle
PMCID: PMC383683  PMID: 111241

Abstract

Mouse-hamster somatic cell hybrids containing a variable number of mouse chromosomes have been used in experiments to determine which mouse chromosome carries the immunoglobulin kappa light chain genes. It has been shown by nucleic acid hybridization that the kappa constant gene and the genes for at least one variable region subgroup are on mouse chromosome 6. This somatic cell genetic mapping procedure appears to be general and can be applied to any expressed or silent gene for which an appropriate nucleic acid probe exists.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Brack C., Hirama M., Lenhard-Schuller R., Tonegawa S. A complete immunoglobulin gene is created by somatic recombination. Cell. 1978 Sep;15(1):1–14. doi: 10.1016/0092-8674(78)90078-8. [DOI] [PubMed] [Google Scholar]
  2. Deisseroth A., Nienhuis A., Lawrence J., Giles R., Turner P., Ruddle F. H. Chromosomal localization of human beta globin gene on human chromosome 11 in somatic cell hybrids. Proc Natl Acad Sci U S A. 1978 Mar;75(3):1456–1460. doi: 10.1073/pnas.75.3.1456. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Deisseroth A., Nienhuis A., Turner P., Velez R., Anderson W. F., Ruddle F., Lawrence J., Creagan R., Kucherlapati R. Localization of the human alpha-globin structural gene to chromosome 16 in somatic cell hybrids by molecular hybridization assay. Cell. 1977 Sep;12(1):205–218. doi: 10.1016/0092-8674(77)90198-2. [DOI] [PubMed] [Google Scholar]
  4. Edelman G. M., Gall W. E. The antibody problem. Annu Rev Biochem. 1969;38:415–466. doi: 10.1146/annurev.bi.38.070169.002215. [DOI] [PubMed] [Google Scholar]
  5. Gottlieb P. D., Durda P. J. The IB-peptide marker and the Ly-3 surface alloantigen: structural studies of a V kappa-region polymorphism and a T-cell marker determined by linked genes. Cold Spring Harb Symp Quant Biol. 1977;41(Pt 2):805–815. doi: 10.1101/sqb.1977.041.01.091. [DOI] [PubMed] [Google Scholar]
  6. Hengartner H., Meo T., Müller E. Assignment of genes for immunoglobulin kappa and heavy chains to chromosomes 6 and 12 in mouse. Proc Natl Acad Sci U S A. 1978 Sep;75(9):4494–4498. doi: 10.1073/pnas.75.9.4494. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Kindt T. J. Rabbit immunoglobulin allotypes: structure, immunology, and genetics. Adv Immunol. 1975;21:35–86. doi: 10.1016/s0065-2776(08)60218-7. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Kozak C. A., Lawrence J. B., Ruddle F. H. A sequential staining technique for the chromosomal analysis of the interspecific mouse/hamster and mouse/human somatic cell hybrids. Exp Cell Res. 1977 Mar 1;105(1):109–117. doi: 10.1016/0014-4827(77)90156-2. [DOI] [PubMed] [Google Scholar]
  9. Laskin J. A., Gray A., Nisonoff A., Klinman N. R., Gottlieb P. D. Segregation at a locus determining an immunoglobulin genetic marker for the light chain variable region affects inheritance of expression of an idiotype. Proc Natl Acad Sci U S A. 1977 Oct;74(10):4600–4604. doi: 10.1073/pnas.74.10.4600. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Leinwand L. A., Kozak C. A., Ruddle F. H. Assignment of the genes for triose phosphate isomerase to chromosome 6 and tripeptidase-1 to chromosome 10 in Mus musculus by somatic cell hybridization. Somatic Cell Genet. 1978 Mar;4(2):233–240. doi: 10.1007/BF01538987. [DOI] [PubMed] [Google Scholar]
  11. Leinwand L., Fournier R. E., Nichols E. A., Ruddle F. H. Assignment of the gene for adenosine kinase to chromosome 14 in Mus musculus by somatic cell hybridization. Cytogenet Cell Genet. 1978;21(1-2):77–85. doi: 10.1159/000130880. [DOI] [PubMed] [Google Scholar]
  12. Miller C. L., Ruddle F. H. Co-transfer of human X-linked markers into murine somatic cells via isolated metaphase chromosomes. Proc Natl Acad Sci U S A. 1978 Jul;75(7):3346–3350. doi: 10.1073/pnas.75.7.3346. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Natvig J. B., Kunkel H. G. Human immunoglobulins: classes, subclasses, genetic variants, and idiotypes. Adv Immunol. 1973;16:1–59. doi: 10.1016/s0065-2776(08)60295-3. [DOI] [PubMed] [Google Scholar]
  14. Orkin S. H., Alter B. P., Altay C., Mahoney M. J., Lazarus H., Hobbins J. C., Nathan D. G. Application of endonuclease mapping to the analysis and prenatal diagnosis of thalassemias caused by globin-gene deletion. N Engl J Med. 1978 Jul 27;299(4):166–172. doi: 10.1056/NEJM197807272990403. [DOI] [PubMed] [Google Scholar]
  15. Polsky F., Edgell M. H., Seidman J. G., Leder P. High capacity gel preparative electrophoresis for purification of fragments of genomic DNA. Anal Biochem. 1978 Jul 1;87(2):397–410. doi: 10.1016/0003-2697(78)90689-9. [DOI] [PubMed] [Google Scholar]
  16. Rigby P. W., Dieckmann M., Rhodes C., Berg P. Labeling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase I. J Mol Biol. 1977 Jun 15;113(1):237–251. doi: 10.1016/0022-2836(77)90052-3. [DOI] [PubMed] [Google Scholar]
  17. Ruddle N. H., Conta B. S., Leinwand L., Kozak C., Ruddle F., Besmer P., Baltimore D. Assignment of the receptor for ecotropic murine leukemia virus to mouse chromosome 5. J Exp Med. 1978 Aug 1;148(2):451–465. doi: 10.1084/jem.148.2.451. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Seidman J. G., Edgell M. H., Leder P. Immunoglobulin light-chain structural gene sequences cloned in a bacterial plasmid. Nature. 1978 Feb 9;271(5645):582–585. doi: 10.1038/271582a0. [DOI] [PubMed] [Google Scholar]
  19. Seidman J. G., Leder A., Edgell M. H., Polsky F., Tilghman S. M., Tiemeier D. C., Leder P. Multiple related immunoglobulin variable-region genes identified by cloning and sequence analysis. Proc Natl Acad Sci U S A. 1978 Aug;75(8):3881–3885. doi: 10.1073/pnas.75.8.3881. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Seidman J. G., Leder A., Nau M., Norman B., Leder P. Antibody diversity. Science. 1978 Oct 6;202(4363):11–17. doi: 10.1126/science.99815. [DOI] [PubMed] [Google Scholar]
  21. Seidman J. G., Leder P. The arrangement and rearrangement of antibody genes. Nature. 1978 Dec 21;276(5690):790–795. doi: 10.1038/276790a0. [DOI] [PubMed] [Google Scholar]
  22. Southern E. M. Detection of specific sequences among DNA fragments separated by gel electrophoresis. J Mol Biol. 1975 Nov 5;98(3):503–517. doi: 10.1016/s0022-2836(75)80083-0. [DOI] [PubMed] [Google Scholar]
  23. Tiemeier D. C., Tilghman S. M., Leder P. Purification and cloning of a mouse ribosomal gene fragment in coliphage lambda. Gene. 1977;2(3-4):173–191. doi: 10.1016/0378-1119(77)90016-6. [DOI] [PubMed] [Google Scholar]
  24. Valbuena O., Marcu K. B., Croce C. M., Huebner K., Weigert M., Perry R. P. Chromosomal locations of mouse immunoglobulin genes. Proc Natl Acad Sci U S A. 1978 Jun;75(6):2883–2887. doi: 10.1073/pnas.75.6.2883. [DOI] [PMC free article] [PubMed] [Google Scholar]

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