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. 1979 Jun;76(6):2960–2963. doi: 10.1073/pnas.76.6.2960

Fetal and adult albumins are indistinguishable by immunological and physicochemical criteria

Nilou Gitzelmann-Cumarasamy *, Richard Gitzelmann †, Kenneth J Wilson ‡, Clive C Kuenzle *
PMCID: PMC383730  PMID: 111248

Abstract

The existence of a functionally immature fetal albumin has been postulated to explain the reduced ability of newborn plasma to bind bilirubin and various drugs. In support of this, cord and adult albumin, isolated by a simple salting-out technique, were reported to differ in electrophoretic and chromatographic properties and in their resistance to alkali and proteolytic enzymes. However, the interpretation of these findings has since been questioned. To resolve this controversy, we have purified to homogeneity human serum albumins from pooled umbilical cord and adult donor plasma. The two albumins were compared and found to be indistinguishable by polyacrylamide gel electrophoresis with and without sodium dodecyl sulfate, as well as by immunoelectrophoresis and double immunodiffusion using specific antibodies against both albumins. Furthermore, the amino acid compositions, the aminoterminal sequence (Asp-Ala-His-Lys-Ser-Glu-Val-Ala-), the carboxy terminus (Leu), and the peptide fingerprints were identical in the two albumins. No significant differences were found by circular dichroism in the ultraviolet (200-350 nm). Binding studies with bilirubin showed association constants of 3.7 ± 0.7 × 107 M-1 for cord and 2.9 ± 0.3 × 107 M-1 for adult albumin, respectively. The circular dichroic spectra of 1:1 bilirubin·albumin complexes showed considerable variation between the batches but were not significantly different. The only difference was found in the fluorescence spectra of the bilirubin·albumin complexes, in which complexes with adult albumin showed only 75% of the relative fluorescence exhibited with cord albumin. The combined results nevertheless strongly indicate that fetal and adult albumins are very similar, if not identical.

Keywords: neonatal medicine, bilirubin binding, immunochemistry, protein structure, spectroscopy

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