Abstract
Most of the somatostatin-like activity from pigeon pancreas was found to correspond to small species with an apparent molecular weight of 1500--2500. This species was isolated under conditions minimizing intermolecular interactions and protease activities. The isolated product was characterized by two somatostatin radioimmunoassays, a bioassay, endgroup determination, and amino acid analysis. The structure of the isolated compound was determined to be H-Ala-Gly-cyclo-(Cys-Lys-Asn-Phe-Phe-Trp-Lys-Thr-Phe-Thr-Ser-Cys)-OH. Additionally, small amounts of des-Ala1-somatostatin, a possible degradation product of pancreatic somatostatin, and a large somatostatin-like species with an apparent molecular weight of 11,000--12,500 were detected. It is concluded that the main somatostatin-like polypeptide isolated from pigeon pancreas is identical to the mammalian hypothalamic tetradecapeptide somatostatin.
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Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Arimura A., Sato H., Dupont A., Nishi N., Schally A. V. Somatostatin: abundance of immunoreactive hormone in rat stomach and pancreas. Science. 1975 Sep 19;189(4207):1007–1009. doi: 10.1126/science.56779. [DOI] [PubMed] [Google Scholar]
- Bennett C. D., Rodkey J. A., Sondey J. M., Hirschmann R. Dihydrofolate reductase: the amino acid sequence of the enzyme from a methotrexate-resistant mutant of Escherichia coli. Biochemistry. 1978 Apr 4;17(7):1328–1337. doi: 10.1021/bi00600a030. [DOI] [PubMed] [Google Scholar]
- Brazeau P., Vale W., Burgus R., Ling N., Butcher M., Rivier J., Guillemin R. Hypothalamic polypeptide that inhibits the secretion of immunoreactive pituitary growth hormone. Science. 1973 Jan 5;179(4068):77–79. doi: 10.1126/science.179.4068.77. [DOI] [PubMed] [Google Scholar]
- Brownstein M., Arimura A., Sato H., Schally A. V., Kizer J. S. The regional distribution of somatostatin in the rat brain. Endocrinology. 1975 Jun;96(6):1456–1461. doi: 10.1210/endo-96-6-1456. [DOI] [PubMed] [Google Scholar]
- Burgus R., Ling N., Butcher M., Guillemin R. Primary structure of somatostatin, a hypothalamic peptide that inhibits the secretion of pituitary growth hormone. Proc Natl Acad Sci U S A. 1973 Mar;70(3):684–688. doi: 10.1073/pnas.70.3.684. [DOI] [PMC free article] [PubMed] [Google Scholar]
- CRESTFIELD A. M., MOORE S., STEIN W. H. The preparation and enzymatic hydrolysis of reduced and S-carboxymethylated proteins. J Biol Chem. 1963 Feb;238:622–627. [PubMed] [Google Scholar]
- Dubois M. P. Immunoreactive somatostatin is present in discrete cells of the endocrine pancreas. Proc Natl Acad Sci U S A. 1975 Apr;72(4):1340–1343. doi: 10.1073/pnas.72.4.1340. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Dupont A., Alvarado-Urbina G. Conversion of big pancreatic somatostatin without peptide bond cleavage into somatostatin tetradecapeptide. Life Sci. 1976 Nov 1;19(9):1431–1433. doi: 10.1016/0024-3205(76)90444-6. [DOI] [PubMed] [Google Scholar]
- Hartley B. S. Strategy and tactics in protein chemistry. Biochem J. 1970 Oct;119(5):805–822. doi: 10.1042/bj1190805f. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Hunkapiller M. W., Hood L. E. Direct microsequence analysis of polypeptides using an improved sequenator, a nonprotein carrier (polybrene), and high pressure liquid chromatography. Biochemistry. 1978 May 30;17(11):2124–2133. doi: 10.1021/bi00604a016. [DOI] [PubMed] [Google Scholar]
- LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
- Rivier J., Brazeau P., Vale W., Guillemin R. Somatostatin analogs. Relative importance of the disulfide bridge and of the Ala-Gly side chain for biological activity. J Med Chem. 1975 Feb;18(2):123–126. doi: 10.1021/jm00236a001. [DOI] [PubMed] [Google Scholar]
- Rodkey J. A., Bennett C. D. Micro-Edman degradation: the use of high pressure liquid chromatography and gas chromatography in the amino terminal sequence determination of 8 nanomoles of dihydrofolate reductase from a mouse sarcoma. Biochem Biophys Res Commun. 1976 Oct 18;72(4):1407–1413. doi: 10.1016/s0006-291x(76)80170-2. [DOI] [PubMed] [Google Scholar]
- Schally A. V., Dupont A., Arimura A., Redding T. W., Nishi N., Linthicum G. L., Schlesinger D. H. Isolation and structure of somatostatin from porcine hypothalami. Biochemistry. 1976 Feb 10;15(3):509–514. doi: 10.1021/bi00648a009. [DOI] [PubMed] [Google Scholar]
- Spiess J., Vale W. Investigation of larger forms of somatostatin in pigeon pancreas and rat brain. Metabolism. 1978 Sep;27(9 Suppl 1):1175–1178. doi: 10.1016/0026-0495(78)90038-0. [DOI] [PubMed] [Google Scholar]
- Tarr G. E., Beecher J. F., Bell M., McKean D. J. Polyquarternary amines prevent peptide loss from sequenators. Anal Biochem. 1978 Feb;84(2):622–7?0=ENG. doi: 10.1016/0003-2697(78)90086-6. [DOI] [PubMed] [Google Scholar]
- Vale W., Brazeau P., Rivier C., Brown M., Boss B., Rivier J., Burgus R., Ling N., Guillemin R. Somatostatin. Recent Prog Horm Res. 1975;31:365–397. doi: 10.1016/b978-0-12-571131-9.50014-1. [DOI] [PubMed] [Google Scholar]
- Vale W., Brazeau P., Rivier C., Brown M., Boss B., Rivier J., Burgus R., Ling N., Guillemin R. Somatostatin. Recent Prog Horm Res. 1975;31:365–397. doi: 10.1016/b978-0-12-571131-9.50014-1. [DOI] [PubMed] [Google Scholar]
- Vale W., Ling N., Rivier J., Villarreal J., Rivier C., Douglas C., Brown M. Anatomic and phylogenetic distribution of somatostatin. Metabolism. 1976 Nov;25(11 Suppl 1):1491–1494. doi: 10.1016/s0026-0495(76)80175-8. [DOI] [PubMed] [Google Scholar]
- Vale W., Rivier J., Ling N., Brown M. Biologic and immunologic activities and applications of somatostatin analogs. Metabolism. 1978 Sep;27(9 Suppl 1):1391–1401. doi: 10.1016/0026-0495(78)90081-1. [DOI] [PubMed] [Google Scholar]
- Woods K. R., Wang K. T. Separation of dansyl-amino acids by polyamide layer chromatography. Biochim Biophys Acta. 1967 Feb 21;133(2):369–370. doi: 10.1016/0005-2795(67)90078-5. [DOI] [PubMed] [Google Scholar]