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. 1979 Jun;76(6):3001–3005. doi: 10.1073/pnas.76.6.3001

Recombination between the defective component of an acute leukemia virus and Rous associated virus O, an endogenous virus of chickens.

P N Tsichlis, J M Coffin
PMCID: PMC383739  PMID: 223167

Abstract

The ability of the defective acute leukemia virus of chickens, MC-29, to participate in recombination was investigated by testing the ability of the MC-29 genome to donate sequences to its helper virus. The endogenous virus Rous associated virus O (RAV-O) was used as a helper for MC-29, and its genome was compared by fingerprinting to that of the original RAV-O. In three separate isolates, it was found that the RAV-O used as helper for MC-29 had acquired new sequences near the 3' and 5' ends of its genome. The new 3' proximal sequences resembled the C region found in exogenous but not endogenous avian oncoviruses, and it probably imparted a higher growth rate to the recombinant as compared to RAV-O. One isolate also showed recombination within the env gene. Because we could exclude the possibility that the recombination was with host cell information or with the original helper of MC-29, we conclude that the acquired sequences were derived from the MC-29 genome, and therefore this replication defective virus is not defective in recombination.

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Selected References

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  1. Baltimore D. Tumor viruses: 1974. Cold Spring Harb Symp Quant Biol. 1975;39(Pt 2):1187–1200. doi: 10.1101/sqb.1974.039.01.137. [DOI] [PubMed] [Google Scholar]
  2. Billeter M. A., Parsons J. T., Coffin J. M. The nucleotide sequence complexity of avian tumor virus RNA. Proc Natl Acad Sci U S A. 1974 Sep;71(9):3560–3564. doi: 10.1073/pnas.71.9.3560. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Bister K., Hayman M. J., Vogt P. K. Defectiveness of avian myelocytomatosis virus MC29: isolation of long-term nonproducer cultures and analysis of virus-specific polypeptide synthesis. Virology. 1977 Oct 15;82(2):431–448. doi: 10.1016/0042-6822(77)90017-4. [DOI] [PubMed] [Google Scholar]
  4. Bister K., Vogt P. K. Genetic analysis of the defectiveness in strain MC29 avian leukosis virus. Virology. 1978 Jul 15;88(2):213–221. doi: 10.1016/0042-6822(78)90278-7. [DOI] [PubMed] [Google Scholar]
  5. Coffin J. M., Champion M., Chabot F. Nucleotide sequence relationships between the genomes of an endogenous and an exogenous avian tumor virus. J Virol. 1978 Dec;28(3):972–991. doi: 10.1128/jvi.28.3.972-991.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Duesberg P. H., Bister K., Vogt P. K. The RNA of avian acute leukemia virus MC29. Proc Natl Acad Sci U S A. 1977 Oct;74(10):4320–4324. doi: 10.1073/pnas.74.10.4320. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Hsu T. W., Sabran J. L., Mark G. E., Guntaka R. V., Taylor J. M. Analysis of unintegrated avian RNA tumor virus double-stranded DNA intermediates. J Virol. 1978 Dec;28(3):810–818. doi: 10.1128/jvi.28.3.810-818.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Hunter E. The mechanism for genetic recombination in the avian retroviruses. Curr Top Microbiol Immunol. 1978;79:295–309. doi: 10.1007/978-3-642-66853-1_7. [DOI] [PubMed] [Google Scholar]
  9. Junghans R. P., Hu S., Knight C. A., Davidson N. Heteroduplex analysis of avian RNA tumor viruses. Proc Natl Acad Sci U S A. 1977 Feb;74(2):477–481. doi: 10.1073/pnas.74.2.477. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Kawai S., Hanafusa H. Recombination between a temperature-sensitive mutant and a deletion mutant of Rous sarcoma virus. J Virol. 1976 Aug;19(2):389–397. doi: 10.1128/jvi.19.2.389-397.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Mellon P., Pawson A., Bister K., Martin G. S., Duesberg P. H. Specific RNA sequences and gene products of MC29 avian acute leukemia virus. Proc Natl Acad Sci U S A. 1978 Dec;75(12):5874–5878. doi: 10.1073/pnas.75.12.5874. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Moscovici C., Moscovici M. G., Jimenez H., Lai M. M., Hayman M. J., Vogt P. K. Continuous tissue culture cell lines derived from chemically induced tumors of Japanese quail. Cell. 1977 May;11(1):95–103. doi: 10.1016/0092-8674(77)90320-8. [DOI] [PubMed] [Google Scholar]
  13. Rosenberg N., Baltimore D. The effect of helper virus on Abelson virus-induced transformation of lymphoid cells. J Exp Med. 1978 Apr 1;147(4):1126–1141. doi: 10.1084/jem.147.4.1126. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Scher C. D. Effect of pseudotype on Abelson virus and Kirsten sarcoma virus-induced leukemia. J Exp Med. 1978 Apr 1;147(4):1044–1053. doi: 10.1084/jem.147.4.1044. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Shank P. R., Hughes S. H., Kung H. J., Majors J. E., Quintrell N., Guntaka R. V., Bishop J. M., Varmus H. E. Mapping unintegrated avian sarcoma virus DNA: termini of linear DNA bear 300 nucleotides present once or twice in two species of circular DNA. Cell. 1978 Dec;15(4):1383–1395. doi: 10.1016/0092-8674(78)90063-6. [DOI] [PubMed] [Google Scholar]
  16. Stéhelin D., Graf T. Avian myelocytomatosis and erythroblastosis viruses lack the transforming gene src of avian sarcoma viruses. Cell. 1978 Apr;13(4):745–750. doi: 10.1016/0092-8674(78)90224-6. [DOI] [PubMed] [Google Scholar]
  17. Tereba A., Skoog L., Vogt P. K. RNA tumor virus specific sequences in nuclear DNA of several avian species. Virology. 1975 Jun;65(2):524–534. doi: 10.1016/0042-6822(75)90057-4. [DOI] [PubMed] [Google Scholar]
  18. Vogt P. K., Friis R. R. An avian leukosis virus related to RSV(O): properties and evidence for helper activity. Virology. 1971 Jan;43(1):223–234. doi: 10.1016/0042-6822(71)90240-6. [DOI] [PubMed] [Google Scholar]
  19. Wang L. H., Duesberg P., Beemon K., Vogt P. K. Mapping RNase T1-resistant oligonucleotides of avian tumor virus RNAs: sarcoma-specific oligonucleotides are near the poly(A) end and oligonucleotides common to sarcoma and transformation-defective viruses are at the poly(A) end. J Virol. 1975 Oct;16(4):1051–1070. doi: 10.1128/jvi.16.4.1051-1070.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]

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