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. 1979 Jun;76(6):3024–3028. doi: 10.1073/pnas.76.6.3024

Evidence for internalization of the recognition site of β-adrenergic receptors during receptor subsensitivity induced by (-)-isoproterenol

De-Maw Chuang 1, E Costa 1
PMCID: PMC383744  PMID: 223168

Abstract

In the supernatant (30,000 × g) of frog erythrocyte homogenates, by using gel filtration we detected a protein that could bind [3H]dihydroalprenolol ([3H]DHA) with high affinity. This binding was greatly enhanced when the erythrocytes were preincubated with (-)-isoproterenol. After various periods of incubation with (-)-isoproterenol, the extent of the increase in the density of [3H]DHA binding sites in the cytosol was paralleled by a proportional decrease in the number of [3H]DHA binding sites in the corresponding pellet; both events peaked after 2-3 hr of incubation with (-)-isoproterenol. The Ka of the (-)-isoproterenol-induced increase in [3H]DHA binding in cytosol and the decrease in this binding in the membrane ranged between 60 and 90 nM. The changes in the cytosol and particulate [3H]DHA binding sites were independent of RNA and protein synthesis. The increase in cytosol binding elicited by (-)-isoproterenol was blocked by exposure of the cells to (-)-alprenolol which per se failed to change the cytosol binding of [3H]DHA. Scatchard analysis revealed that the enhanced [3H]DHA binding to cytosol material was due to a 4-fold increase in the Bmax with little or no change in Kd (≈9 nM). Binding displacement data show that these soluble [3H]DHA binding sites resemble the surface membrane recognition sites. Moreover, the ability of various β-adrenergic agents to increase [3H]DHA binding to cytosol after they were incubated with frog erythrocytes paralleled their affinity for membrane-bound β receptors. These findings support the view that the β-adrenergic receptor desensitization caused by prolonged exposure to (-)-isoproterenol is due, at least in part, to an internalization of the recognition site of β-adrenergic receptors.

Keywords: receptor desensitization, receptor internalization, frog erythrocytes, adenylate cyclase

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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