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. 1979 Jul;76(7):3382–3386. doi: 10.1073/pnas.76.7.3382

Bimane fluorescent labels: labeling of normal human red cells under physiological conditions.

N S Kosower, E M Kosower, G L Newton, H M Ranney
PMCID: PMC383829  PMID: 291011

Abstract

The bimane fluorescent labels, monobromobimane, dibromobimane, and monobromotrimethylammoniobimane, are derivatives of syn-9,10-dioxabimane:1,5-diazabicyclo[3.3.0]octa-3,6-diene-2,8-dione. They efficiently label hemoglobin (reactive thiol groups), membrane proteins, and glutathione of normal human red cells under physiological conditions. Monobromobimane and dibromobimane are effective on intact cells while red cell membranes may be impermeable to the positively charged monobromotrimethylammoniobimane, the latter being effective only on lysed cells. These bimane labels provide a class of labeling agents that may have wide applicability in biological materials.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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