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. 1979 Jul;76(7):3420–3424. doi: 10.1073/pnas.76.7.3420

Human T gamma globin chain is a variant of A gamma chain (A gamma Sardinia).

G Saglio, G Ricco, U Mazza, C Camaschella, P G Pich, A M Gianni, E Gianazza, P G Righetti, B Giglioni, P Comi, M Gusmeroli, S Ottolenghi
PMCID: PMC383837  PMID: 291015

Abstract

Isoelectric focusing, cellulose acetate electrophoresis, and carboxymethylcellulose chromatography in the presence of Nonidet P-40 allow the separation of pure gamma chains into two fractions. Amino acid analysis of their cyanogen bromide fragment 3 (gamma CB3) identifies these fractions as the separated G gamma (Gly-136) and A gamma (Ala-136) globin chains. Fingerprint and amino acid analyses of the gamma Tp9 tryptic peptide from the purified A gamma and G gamma fractions from two different patients demonstrate that the commonly occurring gamma Sardinia variant (gamma 75 isoleucine leads to threonine), also known as T gamma chain, has alanine in position 136. From this analysis we suggest that the T gamma gene is an allele of the A gamma locus (A gamma Sardinia) rather than a third gamma locus.

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