Skip to main content
NCBI home page
Proceedings of the National Academy of Sciences of the United States of America logoLink to Proceedings of the National Academy of Sciences of the United States of America
. 1979 Jul;76(7):3445–3449. doi: 10.1073/pnas.76.7.3445

Occurrence of crossed strand-exchange forms in yeast DNA during meiosis.

L Bell, B Byers
PMCID: PMC383842  PMID: 386340

Abstract

The crossed strand-exchange form (Holliday structure, half chiasma) has been predicted as an intermediate in the genetic recombination of eukaryotes. We report here the detection of this form in the yeast plasmid, 2-micron DNA, isolated during meiosis. Physical mapping has previously suggested that two forms of 2-micron DNA arise because of recombination between inverted repeat regions. After appropriate digestion with restriction endonuclease, a crossed strand-exchange form intermediate in this recombination would yield an X-shaped form resistant to loss by branch migration because of nonhomology in sequences flanking the region of homology. We first generated this X-shaped form artificially by reannealing melted restriction fragments of 2-micron DNA. This enabled us to develop a procedure for the physical separation of the X-shaped form by agarose gel electrophoresis. We then used this electrophoretic procedure to isolate a naturally occurring form of identical structure from the 2-micron DNA of meiotic cells. Electron microscopy demonstrated that the exchange junction had the expected configuration of strands and indicated that the junction occurred within the region of homology.

Full text

PDF
3445

Images in this article

3446Image
on p.3446
3447Image
on p.3447

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Beggs J. D. Transformation of yeast by a replicating hybrid plasmid. Nature. 1978 Sep 14;275(5676):104–109. doi: 10.1038/275104a0. [DOI] [PubMed] [Google Scholar]
  2. Benbow R. M., Zuccarelli A. J., Sinsheimer R. L. Recombinant DNA molecules of bacteriophage phi chi174. Proc Natl Acad Sci U S A. 1975 Jan;72(1):235–239. doi: 10.1073/pnas.72.1.235. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Byers B., Goetsch L. Electron microscopic observations on the meiotic karyotype of diploid and tetraploid Saccharomyces cerevisiae. Proc Natl Acad Sci U S A. 1975 Dec;72(12):5056–5060. doi: 10.1073/pnas.72.12.5056. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Byers B., Shriver K., Goetsch L. The role of spindle pole bodies and modified microtubule ends in the initiation of microtubule assembly in Saccharomyces cerevisiae. J Cell Sci. 1978 Apr;30:331–352. doi: 10.1242/jcs.30.1.331. [DOI] [PubMed] [Google Scholar]
  5. Cameron J. R., Philippsen P., Davis R. W. Analysis of chromosomal integration and deletions of yeast plasmids. Nucleic Acids Res. 1977;4(5):1429–1448. doi: 10.1093/nar/4.5.1429. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Clark-Walker G. D. Isolation of circular DNA from a mitochondrial fraction from yeast. Proc Natl Acad Sci U S A. 1972 Feb;69(2):388–392. doi: 10.1073/pnas.69.2.388. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Clark-Walker G. D., Miklos G. L. Localization and quantification of circular DNA in yeast. Eur J Biochem. 1974 Jan 16;41(2):359–365. doi: 10.1111/j.1432-1033.1974.tb03278.x. [DOI] [PubMed] [Google Scholar]
  8. Guerineau M., Grandchamp C., Slonimski P. P. Circular DNA of a yeast episome with two inverted repeats: structural analysis by a restriction enzyme and electron microscopy. Proc Natl Acad Sci U S A. 1976 Sep;73(9):3030–3034. doi: 10.1073/pnas.73.9.3030. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Hollenberg C. P., Degelmann A., Kustermann-Kuhn B., Royer H. D. Characterization of 2-mum DNA of Saccharomyces cerevisiae by restriction fragment analysis and integration in an Escherichia coli plasmid. Proc Natl Acad Sci U S A. 1976 Jun;73(6):2072–2076. doi: 10.1073/pnas.73.6.2072. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Livingston D. M., Klein H. L. Deoxyribonucleic acid sequence organization of a yeast plasmid. J Bacteriol. 1977 Jan;129(1):472–481. doi: 10.1128/jb.129.1.472-481.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Livingston D. M., Kupfer D. M. Control of Saccharomyces cerevisiae 2microN DNA replication by cell division cycle genes that control nuclear DNA replication. J Mol Biol. 1977 Oct 25;116(2):249–260. doi: 10.1016/0022-2836(77)90215-7. [DOI] [PubMed] [Google Scholar]
  12. Matsumoto L., Kasamatsu H., Pikó L., Vinograd J. Mitochondrial DNA replication in sea urchin oocytes. J Cell Biol. 1974 Oct;63(1):146–159. doi: 10.1083/jcb.63.1.146. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Meselson M. S., Radding C. M. A general model for genetic recombination. Proc Natl Acad Sci U S A. 1975 Jan;72(1):358–361. doi: 10.1073/pnas.72.1.358. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Peterson J. B., Gray R. H., Ris H. Meiotic spindle plaques in Saccharomyces cerevisiae. J Cell Biol. 1972 Jun;53(3):837–841. doi: 10.1083/jcb.53.3.837. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Petes T. D., Williamson D. H. Replicating circular DNA molecules in yeast. Cell. 1975 Mar;4(3):249–253. doi: 10.1016/0092-8674(75)90172-5. [DOI] [PubMed] [Google Scholar]
  16. Potter H., Dressler D. On the mechanism of genetic recombination: electron microscopic observation of recombination intermediates. Proc Natl Acad Sci U S A. 1976 Sep;73(9):3000–3004. doi: 10.1073/pnas.73.9.3000. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Roth R., Halvorson H. O. Sporulation of yeast harvested during logarithmic growth. J Bacteriol. 1969 May;98(2):831–832. doi: 10.1128/jb.98.2.831-832.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. SHERMAN F., ROMAN H. Evidence for two types of allelic recombination in yeast. Genetics. 1963 Feb;48:255–261. doi: 10.1093/genetics/48.2.255. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Sigal N., Alberts B. Genetic recombination: the nature of a crossed strand-exchange between two homologous DNA molecules. J Mol Biol. 1972 Nov 28;71(3):789–793. doi: 10.1016/s0022-2836(72)80039-1. [DOI] [PubMed] [Google Scholar]
  20. Simchen G. Are mitotic functions required in meiosis? Genetics. 1974 Apr;76(4):745–753. doi: 10.1093/genetics/76.4.745. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Thompson B. J., Camien M. N., Warner R. C. Kinetics of branch migration in double-stranded DNA. Proc Natl Acad Sci U S A. 1976 Jul;73(7):2299–2303. doi: 10.1073/pnas.73.7.2299. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Thompson B. J., Escarmis C., Parker B., Slater W. C., Doniger J., Tessman I., Warner R. C. Figure-8 configuration of dimers of S13 and phiX174 replicative form DNA. J Mol Biol. 1975 Feb 5;91(4):409–419. doi: 10.1016/0022-2836(75)90269-7. [DOI] [PubMed] [Google Scholar]
  23. Valenzuela M. S., Inman R. B. Visualization of a novel junction in bacteriophage lambda DNA. Proc Natl Acad Sci U S A. 1975 Aug;72(8):3024–3028. doi: 10.1073/pnas.72.8.3024. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Proceedings of the National Academy of Sciences of the United States of America are provided here courtesy of National Academy of Sciences

RESOURCES