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. 1979 Jul;76(7):3455–3459. doi: 10.1073/pnas.76.7.3455

T lymphocyte response to H-2 mutants: cytotoxic effectors are Ly-1+2+.

P J Wettstein, D W Bailey, L E Mobraaten, J Klein, J A Frelinger
PMCID: PMC383844  PMID: 158760

Abstract

The lymphocyte differentiation (Ly) antigen phenotype of cytotoxic effector T cells specific for H-2 mutant alloantigens was determined. Cytotoxic effectors generated in primary mixed lymphocyte culture and specific for H-2Kba and H-2Dda alloantigens are sensitive to both anti-Ly-1 and anti-Ly-2 serum plus complement. Reconstitution analysis demonstrated that the mutant-specific T cells were Ly-1+2+. These observations and those previously reported, which indicated that H-2K/D mutant-specific T cells proliferating in mixed lymphocyte culture were Ly-1+2+, demonstrated that Ly-1+2+ T cells are immunocompetent. Furthermore, the nature of the stimulating H-2 complex alloantigen determines the Ly phenotype of responsive T cells.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bach F. H., Alter B. J. Alternative pathways of T lymphocyte activation. J Exp Med. 1978 Sep 1;148(3):829–834. doi: 10.1084/jem.148.3.829. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Bailey D. W., Kohn H. I. Inherited histocompatibility changes in progeny of irradiated and unirradiated inbred mice. Genet Res. 1965 Nov;6(3):330–340. doi: 10.1017/s0016672300004225. [DOI] [PubMed] [Google Scholar]
  3. Boyse E. A., Itakura K., Stockert E., Iritani C. A., Miura M. Ly-C: a third locus specifying alloantigens expressed only on thymocytes and lymphocytes. Transplantation. 1971 Mar;11(3):351–353. [PubMed] [Google Scholar]
  4. Boyse E. A., Miyazawa M., Aoki T., Old L. J. Ly-A and Ly-B: two systems of lymphocyte isoantigens in the mouse. Proc R Soc Lond B Biol Sci. 1968 Jun 11;170(1019):175–193. doi: 10.1098/rspb.1968.0032. [DOI] [PubMed] [Google Scholar]
  5. Brown J. L., Nairen R., Natherson S. G. Structural differences between the mouse H-2D products of the mutant B10.D2.M504 (H-2da) and the parental nonmutant strain B10.D2 (H-2d). J Immunol. 1978 Mar;120(3):726–733. [PubMed] [Google Scholar]
  6. Cantor H., Boyse E. A. Lymphocytes as models for the study of mammalian cellular differentiation. Immunol Rev. 1977 Jan;33:105–124. doi: 10.1111/j.1600-065x.1977.tb00364.x. [DOI] [PubMed] [Google Scholar]
  7. Forman J., Klein J. Immunogeneic analysis of H-2 mutations. II. Cellular immunity to the H-2DA mutation. J Immunol. 1975 Sep;115(3):711–715. [PubMed] [Google Scholar]
  8. Hirschberg H., Skare H., Thorsby E. Cell mediated lympholysis: CML. a microplate technique requiring few target cells and employing a new method of supernatant collection. J Immunol Methods. 1977;16(2):131–141. doi: 10.1016/0022-1759(77)90048-5. [DOI] [PubMed] [Google Scholar]
  9. Julius M. H., Simpson E., Herzenberg L. A. A rapid method for the isolation of functional thymus-derived murine lymphocytes. Eur J Immunol. 1973 Oct;3(10):645–649. doi: 10.1002/eji.1830031011. [DOI] [PubMed] [Google Scholar]
  10. Kisielow P., Hirst J. A., Shiku H., Beverley P. C., Hoffman M. K., Boyse E. A., Oettgen H. F. Ly antigens as markers for functionally distinct subpopulations of thymus-derived lymphocytes of the mouse. Nature. 1975 Jan 17;253(5488):219–220. doi: 10.1038/253219a0. [DOI] [PubMed] [Google Scholar]
  11. Mathieson B. J., Sharrow S. O., Campbell P. S., Asofsky R. An Lyt differentiated thymocyte subpopulation detected by flow microfluorometry. Nature. 1979 Feb 8;277(5696):478–480. doi: 10.1038/277478a0. [DOI] [PubMed] [Google Scholar]
  12. Nathenson S. G., Brown J. L., Ewenstein B. M., Nisizawa T., Sears D. W., Freed J. H. Structural differences between parent and variant H-2K glycoproteins from mouse strains carrying H-2 gene mutations. Cold Spring Harb Symp Quant Biol. 1977;41(Pt 1):343–349. doi: 10.1101/sqb.1977.041.01.041. [DOI] [PubMed] [Google Scholar]
  13. Niederhuber J. E., Frelinger J. A., Dugan E., Coutinho A., Shreffler D. C. Effects of anti-Ia serum on mitogenic responses. I. Inhibition of the proliferative response to B cell mitogen, LPS, by specific anti-Ia sera. J Immunol. 1975 Dec;115(6):1672–1676. [PubMed] [Google Scholar]
  14. Peck A. B., Bach F. H. A miniaturized mouse mixed leukocyte culture in serum-free and mouse serum supplemented media. J Immunol Methods. 1973 Oct;3(2):147–163. doi: 10.1016/0022-1759(73)90030-6. [DOI] [PubMed] [Google Scholar]
  15. Rychlíková M., Démant P., Egorov I. K. Mixed lymphocyte reaction caused by an H-2D mutation (congenic strains-B10.D2 new-B10.504). Folia Biol (Praha) 1972;18(5):360–363. [PubMed] [Google Scholar]
  16. Stutman O., Shen F. W., Boyse E. A. Ly phenotype of T cells cytotoxic for syngeneic mouse mammary tumors: evidence for T cell interactions. Proc Natl Acad Sci U S A. 1977 Dec;74(12):5667–5671. doi: 10.1073/pnas.74.12.5667. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Wettstein P. J., Bailey D. W., Mobraaten L. E., Klein J., Frelinger J. A. T-lymphocyte response to H-2 mutants. I. Proliferation is dependent on Ly 1+2+ cells. J Exp Med. 1978 May 1;147(5):1395–1404. doi: 10.1084/jem.147.5.1395. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Widmer M. B., Alter B. J., Bach F. H., Bach M. L. Lymphocyte reactivity to serologically undetected components of the major histocompatibility complex. Nat New Biol. 1973 Apr 25;242(121):239–241. doi: 10.1038/newbio242239a0. [DOI] [PubMed] [Google Scholar]

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