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. 1979 Jul;76(7):3515–3517. doi: 10.1073/pnas.76.7.3515

Detection of glucocorticoid receptors on Friend erythroleukemia cells.

D W Golde, N Bersch, M E Lippman, C Friend
PMCID: PMC383858  PMID: 291019

Abstract

The inhibition of dimethyl sulfoxide-induced differentiation of Friend erythroleukemia cells by steroids led us to examine these cells for the presence of glucocorticoid receptors. Direct assessement of dexamethasone binding revealed high-affinity dexamethasone receptors on the untreated cells. The specific binding of [3H]dexamethasone was dose dependent. At a concentration of 10(-8) M, almost all binding sites were occupied. The mean number of binding sites per cell in two separate experiments was 8045 and 7191, respectively, and the Kd varied between 3.38 and 3.49 x 10(-9) M. Dimethyl sulfoxide treatment led to a decrease in the number of dexamethasone binding sites on the cells induced to differentiate. After 5 days of treatment, the mean number of sites per cell was reduced to 1216 and 896 in two experiments, with a Kd of 5 x 10(-9) M. Dexamethasone treatment resulted in a moderate decrease in the efficiency of colony formation within 72 hr after the cells were plated in methylcellulose. The mechanism of this inhibitory effect is unknown. However, it was also dose dependent and could be abrogated by appropriate concentrations of progesterone or 11-deoxycortisone. These results suggest that the steroid effects on growth and differentiation of the erythroleukemia cells may be mediated via glucocorticoid receptors.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Arndt-Jovin D. J., Ostertag W., Eisen H., Klimek F., Jovin T. M. Studies of cellular differentiation by automated cell separation. Two model systems: Friend virus-transformed cells and Hydra attenuata. J Histochem Cytochem. 1976 Jan;24(1):332–347. doi: 10.1177/24.1.1254928. [DOI] [PubMed] [Google Scholar]
  2. Chui D. H., Liao S. K., Walker K. Fetal erythropoiesis in steel mutant mice. III. Defect in differentiation from BFU-E to CFU-E during early development. Blood. 1978 Mar;51(3):539–547. [PubMed] [Google Scholar]
  3. Eisen H., Bach R., Emery R. Induction of spectrin in erythroleukemic cells transformed by Friend virus. Proc Natl Acad Sci U S A. 1977 Sep;74(9):3898–3902. doi: 10.1073/pnas.74.9.3898. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Eisen H., Nasi S., Georgopoulos C. P., Arndt-Jovin D., Ostertag W. Surface changes in differentiating Friend erythroleukemic cells in culture. Cell. 1977 Apr;10(4):689–695. doi: 10.1016/0092-8674(77)90102-7. [DOI] [PubMed] [Google Scholar]
  5. Friend C., Scher W., Holland J. G., Sato T. Hemoglobin synthesis in murine virus-induced leukemic cells in vitro: stimulation of erythroid differentiation by dimethyl sulfoxide. Proc Natl Acad Sci U S A. 1971 Feb;68(2):378–382. doi: 10.1073/pnas.68.2.378. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Gazitt Y., Deitch A. D., Marks P. A., Rifkind R. A. Cell volume changes in relation to the cell cycle of differentiating erythroleukemic cells. Exp Cell Res. 1978 Dec;117(2):413–420. doi: 10.1016/0014-4827(78)90154-4. [DOI] [PubMed] [Google Scholar]
  7. Golde D. W., Bersch N., Cline M. J. Potentiation of erythropoiesis in vitro by dexamethasone. J Clin Invest. 1976 Jan;57(1):57–62. doi: 10.1172/JCI108269. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Golde D. W., Bersch N., Li C. H. Growth hormone modulation of murine erythroleukemia cell growth in vitro. Proc Natl Acad Sci U S A. 1978 Jul;75(7):3437–3439. doi: 10.1073/pnas.75.7.3437. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Golde D. W., Bersch N., Li C. H. Growth hormone: species-specific stimulation of erythropoiesis in vitro. Science. 1977 Jun 3;196(4294):1112–1113. doi: 10.1126/science.870971. [DOI] [PubMed] [Google Scholar]
  10. Harrison P. R. Analysis of erythropoeisis at the molecular level. Nature. 1976 Jul 29;262(5567):353–356. doi: 10.1038/262353a0. [DOI] [PubMed] [Google Scholar]
  11. Koeffler H. P., Cline M. J., Golde D. W. Erythropoiesis in preleukemia. Blood. 1978 Jun;51(6):1013–1019. [PubMed] [Google Scholar]
  12. Lo S. C., Aft R., Ross J., Mueller G. C. Control of globin gene expression by steroid hormones in differentiating Friend leukemia cells. Cell. 1978 Oct;15(2):447–453. doi: 10.1016/0092-8674(78)90014-4. [DOI] [PubMed] [Google Scholar]
  13. Loritz F., Bernstein A., Miller R. G. Early and late volume changes during erythroid differentiation of cultured Friend leukemic cells. J Cell Physiol. 1977 Mar;90(3):423–437. doi: 10.1002/jcp.1040900306. [DOI] [PubMed] [Google Scholar]
  14. Neifeld J. P., Lippman M. E., Tormey D. C. Steroid hormone receptors in normal human lymphocytes. Induction of glucocorticoid receptor activity by phytohemagglutinin stimulation. J Biol Chem. 1977 May 10;252(9):2972–2977. [PubMed] [Google Scholar]
  15. Rousseau G. G., Baxter J. D., Higgins S. J., Tomkins G. M. Steroid-induced nuclear binding of glucocorticoid receptors in intact hepatoma cells. J Mol Biol. 1973 Sep 25;79(3):539–554. doi: 10.1016/0022-2836(73)90405-1. [DOI] [PubMed] [Google Scholar]
  16. Rousseau G. G., Baxter J. D., Tomkins G. M. Glucocorticoid receptors: relations between steroid binding and biological effects. J Mol Biol. 1972 Jun 14;67(1):99–115. doi: 10.1016/0022-2836(72)90389-0. [DOI] [PubMed] [Google Scholar]
  17. Sassa S. Sequential induction of heme pathway enzymes during erythroid differentiation of mouse Friend leukemia virus-infected cells. J Exp Med. 1976 Feb 1;143(2):305–315. doi: 10.1084/jem.143.2.305. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Scher W., Tsuei D., Sassa S., Price P., Gabelman N., Friend C. Inhibition of dimethyl sulfoxide-stimulated Friend cell erythrodifferentiation by hydrocortisone and other steroids. Proc Natl Acad Sci U S A. 1978 Aug;75(8):3851–3855. doi: 10.1073/pnas.75.8.3851. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Yarbro G. S., Lippman M. E., Johnson G. E., Leventhal B. G. Glucocorticoid receptors in subpopulations of childhood acute lymphocytic leukemia. Cancer Res. 1977 Aug;37(8 Pt 1):2688–2695. [PubMed] [Google Scholar]

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