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. 1979 Jul;76(7):3542–3546. doi: 10.1073/pnas.76.7.3542

Localization of horseradish peroxidase-alpha-bungarotoxin binding in crustacean axonal membrane vesicles and intact axons.

J Chester, T L Lentz, J K Marquis, H G Mautner
PMCID: PMC383864  PMID: 291022

Abstract

A conjugate of alpha-bungarotoxin with horseradish peroxidase was used to visualize alpha-bungarotoxin binding sites at the fine structural level in isolated axonal membrane vesicles from lobster walking leg nerve. These plasma membrane vesicles have previously been shown to exhibit saturable binding of [3H]nicotine and [3H]acetylcholine. Binding of the toxin was identified in the axon plasma membrane and could be blocked by pretreatment with excess free alpha-bungaratoxin or d-tubocurarine. Binding sites for alpha-bungarotoxin were identified by the same technique in sections of intact nerve fibers from both lobster and spider crab and were found to be localized primarily in the axolemma rather than in the Schwann cell membrane.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Balerna M., Fosset M., Chicheportiche R., Romey G., Lazdunski M. Constitution and properties of axonal membranes of crustacean nerves. Biochemistry. 1975 Dec 16;14(25):5500–5511. doi: 10.1021/bi00696a019. [DOI] [PubMed] [Google Scholar]
  2. Carbonetto S. T., Fambrough D. M., Muller K. J. Nonequivalence of alpha-bungarotoxin receptors and acetylcholine receptors in chick sympathetic neurons. Proc Natl Acad Sci U S A. 1978 Feb;75(2):1016–1020. doi: 10.1073/pnas.75.2.1016. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. DETTBARN W. D. Effect of curare on conduction in myelinated, isolated nerve fibres of the frog. Nature. 1960 Jun 11;186:891–892. doi: 10.1038/186891a0. [DOI] [PubMed] [Google Scholar]
  4. DETTBARN W. D. New evidence for the role of acetylcholine in conduction. Biochim Biophys Acta. 1960 Jul 15;41:377–386. doi: 10.1016/0006-3002(60)90034-2. [DOI] [PubMed] [Google Scholar]
  5. Darin De Lorenzo A. J., Brzin M., Dettbarn W. D. Fine structure and organization of nerve fibers and giant axons in Homarus americanus. J Ultrastruct Res. 1968 Sep;24(5):367–384. doi: 10.1016/s0022-5320(68)80043-7. [DOI] [PubMed] [Google Scholar]
  6. Darin de Lorenzo A. J., Dettbarn W. D., Brzin M. Fine structural localization of acetylcholinesterase in single axons. J Ultrastruct Res. 1969 Jul;28(1):27–40. doi: 10.1016/s0022-5320(69)90004-5. [DOI] [PubMed] [Google Scholar]
  7. Denburg J. L. An axon plasma membrane preparation from the walking legs of the lobster Homarus americanus. Biochim Biophys Acta. 1972 Sep 1;282(1):453–458. doi: 10.1016/0005-2736(72)90353-7. [DOI] [PubMed] [Google Scholar]
  8. Denburg J. L., Eldefrawi M. E., O'Brien R. D. Macromolecules from lobster axon membranes that bind cholinergic ligands and local anesthetics (recpetors-procaine-acetylcholine-nicotine-Na + and K + gates). Proc Natl Acad Sci U S A. 1972 Jan;69(1):177–181. doi: 10.1073/pnas.69.1.177. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Dettbarn W. D., Rosenberg P. Effect of ions on the efflux of acetylcholine from peripheral nerve. J Gen Physiol. 1966 Nov;50(2):447–460. doi: 10.1085/jgp.50.2.447. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Hess G. P., Andrews J. P. Functional acetylcholine receptor--electroplax membrane microsacs (vesicles): purification and characterization. Proc Natl Acad Sci U S A. 1977 Feb;74(2):482–486. doi: 10.1073/pnas.74.2.482. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Lentz T. L., Chester J. Localization of acetylcholine receptors in central synapses. J Cell Biol. 1977 Oct;75(1):258–267. doi: 10.1083/jcb.75.1.258. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Lentz T. L., Mazurkiewicz J. E., Rosenthal J. Cytochemical localization of acetylcholine receptors at the neuromuscular junction by means of horseradish peroxidase-labeled alpha-bungarotoxin. Brain Res. 1977 Sep 2;132(3):423–442. doi: 10.1016/0006-8993(77)90192-5. [DOI] [PubMed] [Google Scholar]
  13. Marquis J. K., Hilt D. C., Mautner H. G. Direct binding studies of 125 I-alpha-bungarotoxin and 3H-quinuclidinyl benzilate interaction with axon plasma membrane fragments. Evidence for nicotinic and muscarinic binding sites. Biochem Biophys Res Commun. 1977 Sep 23;78(2):476–482. doi: 10.1016/0006-291x(77)90203-0. [DOI] [PubMed] [Google Scholar]
  14. Marquis J. K., Hilt D. C., Papadeas V. A., Mautner H. G. Interaction of cholinergic ligands and local anesthetics with plasma membrane fragments from lobster axon. Proc Natl Acad Sci U S A. 1977 Jun;74(6):2278–2282. doi: 10.1073/pnas.74.6.2278. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. NACHMANSOHN D. Metabolism and function of the nerve cell. Harvey Lect. 1953;49:57–99. [PubMed] [Google Scholar]
  16. Patrick J., Stallcup W. B. Immunological distinction between acetylcholine receptor and the alpha-bungarotoxin-binding component on sympathetic neurons. Proc Natl Acad Sci U S A. 1977 Oct;74(10):4689–4692. doi: 10.1073/pnas.74.10.4689. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Rawlins F. A., Villegas J. Autoradiographic localization of acetylcholine receptors in the Schwann cell membrane of the squid nerve fiber. J Cell Biol. 1978 May;77(2):371–376. doi: 10.1083/jcb.77.2.371. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Rosenberg P., Mautner H. G. Acetylcholine receptor: similarity in axons and junctions. Science. 1967 Mar 24;155(3769):1569–1571. doi: 10.1126/science.155.3769.1569. [DOI] [PubMed] [Google Scholar]
  19. Yazulla S., Schmidt J. Two types of receptors for alpha-bungarotoxin in the synaptic layers of the pigeon retina. Brain Res. 1977 Dec 9;138(1):45–57. doi: 10.1016/0006-8993(77)90783-1. [DOI] [PubMed] [Google Scholar]

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