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. Author manuscript; available in PMC: 2014 Aug 1.
Published in final edited form as: Neuropharmacology. 2013 Mar 28;71:10.1016/j.neuropharm.2013.03.013. doi: 10.1016/j.neuropharm.2013.03.013

Figure 5.

Figure 5

Effect of chronic restraint stress (CRS; 20 min/day) as compared to daily 20 sec tail-lifting and home cage control (CTRL) conditions for 14 days on 5-HT-like immunoreactivity (5-HT-LI) in adrenal glands (AG) and tryptophan hydroxylase (TPH) protein in the hypothalamic paraventricular nucleus (PVN) and adrenal glands (AG). In AG from CTRL animals, scarce 5-HT-LI was sparsely observed in outer cortical areas. Interestingly, in AG from CRS animals, 5-HT-LI strongly increased in the form of large clusters clearly and predominantly observed in outer cortical areas corresponding to the zona glomerulosa and the zona fasciculata. No TPH protein was detected in whole adrenals from both animals groups (E). Immunoblotting of TPH occurred in the PVN but this was unaffected by CRS (E). A positive control for TPH protein detection employed dorsal raphe nucleus (DRN) samples from CTRL animals (E). Bars in A and C = 200 ìm; bars in B and D = 100ìm.