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. Author manuscript; available in PMC: 2014 Jul 23.
Published in final edited form as: Biochemistry. 2013 Jul 8;52(29):10.1021/bi400338f. doi: 10.1021/bi400338f

Figure 2.

Figure 2

pH dependence of ProDH. kcat(app) (●) and kcat/KL-pro(app) (▲), determined at saturating DCPIP and varying L-proline concentrations. The solid lines are fits to eq 3, yielding a pKa of 6.8 ± 0.1 for kcat(app) and 7.1 ± 0.2 for kcat/KL-pro(app). The dashed line in the kcat/KL-pro pH profile represents a fit where two groups must be deprotonated.