Skip to main content
NCBI home page
Proceedings of the National Academy of Sciences of the United States of America logoLink to Proceedings of the National Academy of Sciences of the United States of America
. 1979 Aug;76(8):3880–3884. doi: 10.1073/pnas.76.8.3880

Induction of cytokinin autonomy by N,N′-diphenylurea in tissue cultures of Phaseolus lunatus L

Machteld C Mok *, Sang-Gu Kim †, Donald J Armstrong †, David W S Mok *
PMCID: PMC383939  PMID: 16592694

Abstract

The ability of N,N′-diphenylurea (Ph2urea) to substitute for cytokinin-active adenine derivatives in promoting callus growth of Phaseolus lunatus has been examined. In general, Ph2urea stimulated callus growth at high concentrations, although the growth of most callus tissues was irregular. Variability in the sensitivity and uniformity of the growth response to Ph2urea was found among different genotypes of P. lunatus. Most importantly, tissues cultured on Ph2urea-containing medium for one passage had acquired the ability to proliferate in subsequent passages in the absence of either Ph2urea or cytokinin-active adenine derivatives. Corresponding tissues maintained on kinetin-containing medium remained cytokinin-dependent. It appears that the effect of Ph2urea in promoting the growth of P. lunatus callus tissue resides in its ability to induce cytokinin autonomy. This result suggests that the cytokinin activity of Ph2urea may be due to promotion of endogenous cytokinin biosynthesis in the bioassay systems in which it is active.

Keywords: plant hormones, genetic variation, bean callus

Full text

PDF
3880

Images in this article

3881Image
on p.3881
3882Image
on p.3882
3883Image
on p.3883

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bednar T. W., Linsmaier-Bednar E. M. Induction of cytokinin-independent tobacco tissues by substituted fluorenes. Proc Natl Acad Sci U S A. 1971 Jun;68(6):1178–1179. doi: 10.1073/pnas.68.6.1178. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Bednar T. W., Linsmaier-Bednar E. M. Induction of plant tissue neoplasms in vitro by substituted fluorenes. Chem Biol Interact. 1972 Mar;4(4):233–238. doi: 10.1016/0009-2797(72)90018-x. [DOI] [PubMed] [Google Scholar]
  3. Bruce M. I., Zwar J. A. Cytokinin activity of some substituted ureas and thioureas. Proc R Soc Lond B Biol Sci. 1966 Aug 16;165(999):245–265. doi: 10.1098/rspb.1966.0067. [DOI] [PubMed] [Google Scholar]
  4. Burrows W. J., Leworthy D. P. Metabolism of N,N'-diphenylurea by cytokinin-dependent tobacco callus: identification of the glucoside. Biochem Biophys Res Commun. 1976 Jun 21;70(4):1109–1114. doi: 10.1016/0006-291x(76)91017-2. [DOI] [PubMed] [Google Scholar]
  5. Dyson W. H., Fox J. E., McChesney J. D. Short term metabolism of urea and purine cytokinins. Plant Physiol. 1972 Apr;49(4):506–513. doi: 10.1104/pp.49.4.506. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Peterson J. B., Miller C. O. Cytokinins in Vinca rosea L. Crown Gall Tumor Tissue as Influenced by Compounds Containing Reduced Nitrogen. Plant Physiol. 1976 Mar;57(3):393–399. doi: 10.1104/pp.57.3.393. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Schmitz R. Y., Skoog F. The use of dimethylsulfoxide as a solvent in the tobacco bioassay for cytokinins. Plant Physiol. 1970 Apr;45(4):537–538. doi: 10.1104/pp.45.4.537. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Proceedings of the National Academy of Sciences of the United States of America are provided here courtesy of National Academy of Sciences

RESOURCES