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Proceedings of the National Academy of Sciences of the United States of America logoLink to Proceedings of the National Academy of Sciences of the United States of America
. 1979 Aug;76(8):4016–4019. doi: 10.1073/pnas.76.8.4016

Human cell lines expressing intestinal alkaline phosphatase.

F J Benham, H Harris
PMCID: PMC383967  PMID: 291061

Abstract

At least three loci determine human alkaline phosphatases [orthophosphoric-monoester phosphohydrolase (alkaline optimum), EC 3.1.3.1]: one coding for the placental form of the enzyme, at least one coding for the intestinal forms, and at least one for the liver, bone, and kidney forms. The alkaline phosphatase in cell line D98/AH-2 has been characterized by inhibition, thermostability, and electrophoretic studies. It is intestinal in type and resembles the fetal intestinal form somewhat more closely than the adult intestinal form. Intestinal alkaline phosphatase was found in the related cell lines Detroit 98, D98/S, and D98/AH-R. No placental alkaline phosphatase could be detected in any of these cell lines. This series of cell lines are believed, on the basis of earlier investigations, to be HeLa in origin but other HeLa cell lines show placental alkaline phosphatase. Loss of expression of the placental alkaline phosphatase locus probably occurred prior to the separation of Detroit-98 from the lineage leading to other HeLa cell lines and this has persisted in the Detroit-98 derivatives D98/AH-2, D98/S, and D98/AH-R. Another possibility is that placental alkaline phosphatase expression only appeared in the HeLa lineage subsequent to the separation of Detroit-98.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Beckman G., Beckman L., Lundgren E. Isozyme variations in human cells grown in vitro. IV. Identity between alkaline phosphatases from HeLa cells and placenta? Hum Hered. 1970;20(2):182–186. doi: 10.1159/000152308. [DOI] [PubMed] [Google Scholar]
  2. Benham F. J., Povey M. S., Harris H. Heterogeneity of alkaline phosphatases in different HeLa lines. Somatic Cell Genet. 1978 Jan;4(1):13–25. doi: 10.1007/BF01546490. [DOI] [PubMed] [Google Scholar]
  3. Benham F. J., Povey M. S., Harris H. Placental-like alkaline phosphatase in malignant and benign ovarian tumors. Clin Chim Acta. 1978 Jun;86(2):201–215. doi: 10.1016/0009-8981(78)90134-1. [DOI] [PubMed] [Google Scholar]
  4. Elson N. A., Cox R. P. Production of fetal-like alkaline phosphatase by HeLa cells. Biochem Genet. 1969 Dec;3(6):549–561. doi: 10.1007/BF00485476. [DOI] [PubMed] [Google Scholar]
  5. Fishman W. H., Inglis N. R., Green S., Anstiss C. L., Gosh N. K., Reif A. E., Rustigian R., Krant M. J., Stolbach L. L. Immunology and biochemistry of Regan isoenzyme of alkaline phosphatase in human cancer. Nature. 1968 Aug 17;219(5155):697–699. doi: 10.1038/219697a0. [DOI] [PubMed] [Google Scholar]
  6. Fishman W. H., Sie H. G. Organ-specific inhibition of human alkaline phosphatase isoenzymes of liver, bone, intestine and placenta; L-phenylalanine, L-tryptophan and L homoarginine. Enzymologia. 1971 Sep 30;41(3):141–167. [PubMed] [Google Scholar]
  7. Gartler S. M. Apparent Hela cell contamination of human heteroploid cell lines. Nature. 1968 Feb 24;217(5130):750–751. doi: 10.1038/217750a0. [DOI] [PubMed] [Google Scholar]
  8. Gartler S. M. Genetic markers as tracers in cell culture. Natl Cancer Inst Monogr. 1967 Sep;26:167–195. [PubMed] [Google Scholar]
  9. Greene P. J., Sussman H. H. Structual comparison of ectopic and normal placental alkaline phosphatase. Proc Natl Acad Sci U S A. 1973 Oct;70(10):2936–2940. doi: 10.1073/pnas.70.10.2936. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Higashino K., Kudo S., Ohtani R., Yamamura Y. Further observation of Kashara isoenzyme in patients with malignant diseases. Gan. 1976 Dec;67(6):909–911. [PubMed] [Google Scholar]
  11. Higashino K., Otani R., Kudo S., Hashinostume M., Hada T. Hepatocellular carcinoma and a variant alkaline phosphatase. Ann Intern Med. 1975 Jul;83(1):74–78. doi: 10.7326/0003-4819-83-1-74. [DOI] [PubMed] [Google Scholar]
  12. Higashino K., Otani R., Kudo S., Yamamura Y. A fetal intestinal-type alkaline phosphatase in hepatocellular carcinoma tissue. Clin Chem. 1977 Sep;23(9):1615–1623. [PubMed] [Google Scholar]
  13. Khattab M., Pfleiderer G. Alkaline phosphatase of human and calf small intestine. Purification and immunochemical characterization. Hoppe Seylers Z Physiol Chem. 1976 Mar;357(3):377–391. doi: 10.1515/bchm2.1976.357.1.377. [DOI] [PubMed] [Google Scholar]
  14. Komoda T., Sakagishi Y. Partial purification of human intestinal alkaline phosphatase with affinity chromotography. Some properties and interaction of concanavalin A with alkaline phosphatase. Biochim Biophys Acta. 1976 Oct 11;445(3):645–660. doi: 10.1016/0005-2744(76)90117-0. [DOI] [PubMed] [Google Scholar]
  15. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  16. Lavappa K. S., Macy M. L., Shannon J. E. Examination of ATCC stocks for HeLa marker chromosomes in human cell lines. Nature. 1976 Jan 22;259(5540):211–213. doi: 10.1038/259211a0. [DOI] [PubMed] [Google Scholar]
  17. Lehmann F. G. Immunological relationship between human placental and intestinal alkaline phosphatase. Clin Chim Acta. 1975 Dec 15;65(3):257–269. doi: 10.1016/0009-8981(75)90251-x. [DOI] [PubMed] [Google Scholar]
  18. Moss D. W. Alkaline phosphatase isoenzymes. Technical and clinical aspects. Enzyme. 1975;20(1):20–34. doi: 10.1159/000458916. [DOI] [PubMed] [Google Scholar]
  19. Mulivor R. A., Hannig V. L., Harris H. Developmental change in human intestinal alkaline phosphatase. Proc Natl Acad Sci U S A. 1978 Aug;75(8):3909–3912. doi: 10.1073/pnas.75.8.3909. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Mulivor R. A., Mennuti M., Zackai E. H., Harris H. Prenatal diagnosis of hypophosphatasia; genetic, biochemical, and clinical studies. Am J Hum Genet. 1978 May;30(3):271–282. [PMC free article] [PubMed] [Google Scholar]
  21. Mulivor R. A., Plotkin L. I., Harris H. Differential inhibition of the products of the human alkaline phosphatase loci. Ann Hum Genet. 1978 Jul;42(1):1–13. doi: 10.1111/j.1469-1809.1978.tb00927.x. [DOI] [PubMed] [Google Scholar]
  22. NEALE F. C., CLUBB J. S., HOTCHKIS D., POSEN S. HEAT STABILITY OF HUMAN PLACENTAL ALKALINE PHOSPHATASE. J Clin Pathol. 1965 May;18:359–363. doi: 10.1136/jcp.18.3.359. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Nathanson L., Fishman W. H. New observations on the Regan isoenzyme of alkaline phosphatase in cancer patients. Cancer. 1971 Jun;27(6):1388–1397. doi: 10.1002/1097-0142(197106)27:6<1388::aid-cncr2820270619>3.0.co;2-0. [DOI] [PubMed] [Google Scholar]
  24. Nelson-Rees W. A., Flandermeyer R. R. HeLa cultures defined. Science. 1976 Jan 9;191(4222):96–98. doi: 10.1126/science.1246601. [DOI] [PubMed] [Google Scholar]
  25. Povey S., Hopkinson D. A., Harris H., Franks L. M. Characterisation of human cell lines and differentiation from HeLa by enzyme typing. Nature. 1976 Nov 4;264(5581):60–63. doi: 10.1038/264060b0. [DOI] [PubMed] [Google Scholar]
  26. Stolbach L. L., Krant M. J., Fishman W. H. Ectopic production of an alkaline phosphatase isoenzyme in patients with cancer. N Engl J Med. 1969 Oct 2;281(14):757–762. doi: 10.1056/NEJM196910022811403. [DOI] [PubMed] [Google Scholar]
  27. Sussman H. H., Small P. A., Jr, Cotlove E. Human alkaline phosphatase. Immunochemical identification of organ-specific isoenzymes. J Biol Chem. 1968 Jan 10;243(1):160–166. [PubMed] [Google Scholar]
  28. Suzuki H., Iino S., Endo Y., Torii M., Miki K. Tumor-specific alkaline phosphatase in hepatoma. Ann N Y Acad Sci. 1975 Aug 22;259:307–320. doi: 10.1111/j.1749-6632.1975.tb25427.x. [DOI] [PubMed] [Google Scholar]
  29. Tan K. K., Aw S. E. The purification and properties of heat-stable alkaline phosphatase isoenzymes from HeLa cells. Biochim Biophys Acta. 1971 Apr 14;235(1):119–127. doi: 10.1016/0005-2744(71)90039-8. [DOI] [PubMed] [Google Scholar]
  30. Vanneuville F. J., Van Elsen A. F., Leroy J. G. Alkaline phosphatase: presence and behaviour in human diploid fibroblasts. Arch Int Physiol Biochim. 1975 Dec;83(5):1017–1018. [PubMed] [Google Scholar]
  31. Vanneuville F. J., Van Elsen A. F., Leroy J. G. Alkaline phosphatase: properties in crude homogenates of human diploid fibroblasts [proceedings]. Biochem Soc Trans. 1977;5(4):1117–1117. doi: 10.1042/bst0051117. [DOI] [PubMed] [Google Scholar]
  32. Warnock M. L., Reisman R. Variant alkaline phosphatase in human hepatocellular cancers. Clin Chim Acta. 1969 Apr;24(1):5–11. doi: 10.1016/0009-8981(69)90134-x. [DOI] [PubMed] [Google Scholar]
  33. Whitby L. G., Moss D. W. Analysis of heat inactivation curves of alkaline phosphatase isoenzymes in serum. Clin Chim Acta. 1975 Mar 24;59(3):361–367. doi: 10.1016/0009-8981(75)90012-1. [DOI] [PubMed] [Google Scholar]

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