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. 1983 Jul;80(14):4441–4445. doi: 10.1073/pnas.80.14.4441

Drosophila locus with gene-dosage effects on rhodopsin

N J Scavarda 1,*, J O'Tousa 1, W L Pak 1
PMCID: PMC384054  PMID: 16593338

Abstract

Mutations that decrease the amplitude of the prolonged depolarizing afterpotential (PDA) in Drosophila melanogaster have been shown to have reduced rhodopsin content in the rhabdomeres of photoreceptor cells. In the present study, a genetic analysis of a class of third chromosome PDA-defective mutants localized the ninaE locus to the salivary band region 92A-B. In flies with only one copy of this region instead of the normal two copies, and in ninaE heterozygotes, the rhodopsin content of the major class of photoreceptors is reduced. Three doses of this region increase the rhodopsin content of these photoreceptors. These characteristics of the ninaE locus are expected of the structural gene encoding the major species of opsin in the Drosophila compound eye.

Keywords: vision, mutations, structural gene, in vivo microspectrophotometry, prolonged depolarizing potential

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Alawi A. A., Pak W. L. On-transient of insect electroretinogram: its cellular origin. Science. 1971 Jun 4;172(3987):1055–1057. doi: 10.1126/science.172.3987.1055. [DOI] [PubMed] [Google Scholar]
  2. BROWN K. T., MURAKAMI M. A NEW RECEPTOR POTENTIAL OF THE MONKEY RETINA WITH NO DETECTABLE LATENCY. Nature. 1964 Feb 8;201:626–628. doi: 10.1038/201626a0. [DOI] [PubMed] [Google Scholar]
  3. Bingham P. M., Levis R., Rubin G. M. Cloning of DNA sequences from the white locus of D. melanogaster by a novel and general method. Cell. 1981 Sep;25(3):693–704. doi: 10.1016/0092-8674(81)90176-8. [DOI] [PubMed] [Google Scholar]
  4. Brown H. M., Cornwall M. C. Ionic mechanism of a quasi-stable depolarization in barnacle photoreceptor following red light. J Physiol. 1975 Jul;248(3):579–593. doi: 10.1113/jphysiol.1975.sp010989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Cosens D., Wright R. Light elicited isolation of the complementary visual input systems in white-eye Drosophila. J Insect Physiol. 1975 May;21(5):1111–1120. doi: 10.1016/0022-1910(75)90124-9. [DOI] [PubMed] [Google Scholar]
  6. Eichenbaum D. M., Goldsmith T. H. Propertiesof intact photoreceptor cells lacking synapses. J Exp Zool. 1968 Sep;169(1):15–32. doi: 10.1002/jez.1401690104. [DOI] [PubMed] [Google Scholar]
  7. Harris W. A., Ready D. F., Lipson E. D., Hudspeth A. J., Stark W. S. Vitamin A deprivation and Drosophila photopigments. Nature. 1977 Apr 14;266(5603):648–650. doi: 10.1038/266648a0. [DOI] [PubMed] [Google Scholar]
  8. Hochstein S., Minke B., Hillman P. Antagonistic components of the late receptor potential in the barnacle photoreceptor arising from different stages of the pigment process. J Gen Physiol. 1973 Jul;62(1):105–128. doi: 10.1085/jgp.62.1.105. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Larrivee D. C., Conrad S. K., Stephenson R. S., Pak W. L. Mutation that selectively affects rhodopsin concentration in the peripheral photoreceptors of Drosophila melanogaster. J Gen Physiol. 1981 Nov;78(5):521–545. doi: 10.1085/jgp.78.5.521. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Lindsley D. L., Sandler L., Baker B. S., Carpenter A. T., Denell R. E., Hall J. C., Jacobs P. A., Miklos G. L., Davis B. K., Gethmann R. C. Segmental aneuploidy and the genetic gross structure of the Drosophila genome. Genetics. 1972 May;71(1):157–184. doi: 10.1093/genetics/71.1.157. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Muijser H., Leutscher-Hazelhoff J. T., Stavenga D. G., Kuiper J. W. Photopigment conversions expressed in receptor potential and membrane resistance of blowfly visual sense cells. Nature. 1975 Apr 10;254(5500):520–522. doi: 10.1038/254520a0. [DOI] [PubMed] [Google Scholar]
  12. Nolte J., Brown J. E. Ultraviolet-induced sensitivity to visible light in ultraviolet receptors of Limulus. J Gen Physiol. 1972 Feb;59(2):186–200. doi: 10.1085/jgp.59.2.186. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Ostroy S. E. Characteristics of Drosophila rhodopsin in wild-type and norpA vision transduction mutants. J Gen Physiol. 1978 Nov;72(5):717–732. doi: 10.1085/jgp.72.5.717. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Ostroy S. E., Wilson M., Pak W. L. Drosophila rhodopsin: photochemistry, extraction and differences in the norp AP12 phototransduction mutant. Biochem Biophys Res Commun. 1974 Aug 5;59(3):960–966. doi: 10.1016/s0006-291x(74)80073-2. [DOI] [PubMed] [Google Scholar]
  15. Pak W. L., Conrad S. K., Kremer N. E., Larrivee D. C., Schinz R. H., Wong F. Photoreceptor function. Basic Life Sci. 1980;16:331–346. doi: 10.1007/978-1-4684-7968-3_24. [DOI] [PubMed] [Google Scholar]
  16. Pak W. L., Lidington K. J. Fast electrical potential from a long-lived, long-wavelength photoproduct of fly visual pigment. J Gen Physiol. 1974 Jun;63(6):740–756. doi: 10.1085/jgp.63.6.740. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Pak W. L. Rapid photoresponses in the retina and their relevance to vision research. Photochem Photobiol. 1968 Nov;8(5):495–503. doi: 10.1111/j.1751-1097.1968.tb05893.x. [DOI] [PubMed] [Google Scholar]
  18. Scalenghe F., Turco E., Edström J. E., Pirrotta V., Melli M. Microdissection and cloning of DNA from a specific region of Drosophila melanogaster polytene chromosomes. Chromosoma. 1981;82(2):205–216. doi: 10.1007/BF00286105. [DOI] [PubMed] [Google Scholar]
  19. Schinz R. H., Lo M. V., Larrivee D. C., Pak W. L. Freeze-fracture study of the Drosophila photoreceptor membrane: mutations affecting membrane particle density. J Cell Biol. 1982 Jun;93(3):961–967. doi: 10.1083/jcb.93.3.961. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Stark S. W., Ivanyshyn A. M., Hu K. G. Spectral sensitivities and photopigments in adaptation of fly visual receptors. Naturwissenschaften. 1976 Nov;63(11):513–518. doi: 10.1007/BF00596847. [DOI] [PubMed] [Google Scholar]
  21. Stephenson R. S., Pak W. L. Heterogenic components of a fast electrical potential in Drosophila compound eye and their relation to visual pigment photoconversion. J Gen Physiol. 1980 Apr;75(4):353–379. doi: 10.1085/jgp.75.4.353. [DOI] [PMC free article] [PubMed] [Google Scholar]

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