Table 2.
Interacting proteins retrieved with Biotin-Azo-UbVME and identified by MS
| MW (kDa) |
Uninfected HeLa cells (unique peptides/sequence converage) |
|||
|---|---|---|---|---|
| Protein | Accession Number (NCBI) | pellet | supernatant | |
| Proteasome subunits | ||||
| alpha subunits | ||||
| PSMA1 | 23110935 | 30 | 4/15% | 34/44% |
| beta subunits | ||||
| PSMB5 | 4506201 | 28 | 2/8.7% | 2/7.6% |
| 26S ATPase | ||||
| PSMC1; p56; S4 | 24430151 | 49 | 13/31% | 20/45% |
| PSMC2; MSS1; S7 | 4506209 | 49 | 16/42% | 25/48% |
| PSMC3; TBP1 | 21361144 | 49 | 13/36% | 28/62% |
| PSMC4; MIP224; S6 | 5729991 | 47 | 8/23% | 22/39% |
| PSMC5; p45; S8 | 312596881 | 45 | 15/48% | 27/69% |
| PSMC6; p42 | 195539395 | 46 | 17/49% | 18/50% |
| 26S non-ATPase | ||||
| PSMD1; P112; S1 | 25777600 | 106 | 16/23% | 34/44% |
| PSMD2; P97; S2 | 25777602 | 100 | 23/30% | 28/39% |
| PSMD3; P58; S3 | 25777612 | 61 | 15/30% | 17/31% |
| PSMD4; S5A | 5292161 | 41 | 16/41% | 15/45% |
| PSMD5; S5B | 4826952 | 56 | 3/7.1% | 16/38% |
| PSMD6; S10 | 7661914 | 46 | 9/28% | 9/26% |
| PSMD7; P40; S12 | 25777615 | 37 | 12/43% | 10/40% |
| PSMD8; p31; S14 | 156631005 | 40 | 4/14% | 2/7.1% |
| PSMD9; p27 | 18543329 | 25 | 5/23% | 7/34% |
| PSMD10; p28 | 4506217 | 24 | 6/35% | |
| PSMD11; p44.5; S9 | 28872725 | 47 | 11/28% | 14/35% |
| PSMD12; p55 | 4506221 | 53 | 6/12% | 9/19% |
| PSMD13; p40.5; S11 | 157502193 | 43 | 7/21% | 5/14% |
| PSMD14 | 5031981 | 35 | 3/16% | 5/21% |
| ADRM1; hRpn13 | 28373192 (+1) | 42 | 12/19% | 14/25% |
| Proteasome associated | ||||
| PAAF1; PAAF; WDR71 | 13376751 | 42 | 10/28% | 8/18% |
| Other interactors | ||||
| HCF-C1 | 98986457 | 209 | 15/4.9% | 1/1.2% |
| galectin-7 | 109948279 (+1) | 15 | 5/45% | |
| VCP; p97 | 6005942 | 89 | 31/43% | 13/22% |
| G3BP-1 | 38327552 (+1) | 52 | 8/18% | 10/21% |
| G3BP-2 | 19923399 (+1) | 54 | 4/9.8% | 6/12% |
| WD repeat-containing protein 48 | 18874090 | 76 | 33/47% | 26/41% |
| WD repeat-containing protein 20 isoform 7 | 334848139 | 67 | 6/13% | 4/8.5% |
| thioredoxin-like protein 1 | 4759274 | 32 | 13/55% | |