Abstract
Twelve cell lines representing 10 genera of three orders (Diptera, Lepidoptera, and Orthoptera) of the class Insecta and one cell line (Acarina) from the class Arachnida were examined to discern their sensitivity to the lethal effects of x-irradiation. Radiosensitivity was measured by a combination of colony formation and population growth curve techniques. Each of these arthropod cell lines is significantly more radioresistant than mammalian cells, though the degree of resistance varies greatly with order. Dipteran cells are 3 to 9 times and lepidopteran cells 52 to 104 times more radioresistant than mammalian cells. Orthopteran and acarine cells are intermediate in radiosensitivity between dipteran and lepidopteran cells. These cells, especially the lepidopteran, should be valuable in determining the molecular nature of repair mechanisms that result in resistance to ionizing radiation.
Keywords: cell survival, growth curves, colony formation, cell culture, cellular repair processes
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Selected References
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- Boyd J. B., Setlow R. B. Characterization of postreplication repair in mutagen-sensitive strains of Drosophila melanogaster. Genetics. 1976 Nov;84(3):507–526. doi: 10.1093/genetics/84.3.507. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Ducoff H. S. Causes of death in irradiated adult insects. Biol Rev Camb Philos Soc. 1972 May;47(2):211–240. doi: 10.1111/j.1469-185x.1972.tb00973.x. [DOI] [PubMed] [Google Scholar]
- Ennis T. J., Sohi S. S. Chromosomal characterisation of five lepidopteran cell lines of Malacosoma disstria (Lasiocampidae) and Christoneura fumiferana (Tortricidae). Can J Genet Cytol. 1976 Sep;18(3):471–477. doi: 10.1139/g76-057. [DOI] [PubMed] [Google Scholar]
- HUGHES-SCHRADER S., SCHRADER F. The kinetochore of the Hemiptera. Chromosoma. 1961;12:327–350. doi: 10.1007/BF00328928. [DOI] [PubMed] [Google Scholar]
- Koval T. M., Hart R. W., Myser W. C., Hink W. F. A comparison of survival and repair of UV-induced DNA damage in cultured insect versus mammalian cells. Genetics. 1977 Nov;87(3):513–518. doi: 10.1093/genetics/87.3.513. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Koval T. M., Hart R. W., Myser W. C., Hink W. F. DNA single-strand break repair in cultured insect and mammalian cells after X-irradiation. Int J Radiat Biol Relat Stud Phys Chem Med. 1979 Feb;35(2):183–188. doi: 10.1080/09553007914550201. [DOI] [PubMed] [Google Scholar]
- Koval T. M., Myser W. C., Hart R. W., Hink W. F. Comparison of survival and unscheduled DNA synthesis between an insect and a mammalian cell line following X-ray treatments. Mutat Res. 1978 Mar;49(3):431–435. doi: 10.1016/0027-5107(78)90114-8. [DOI] [PubMed] [Google Scholar]
- Murakami A., Imai H. T. Cytological evidence for holocentric chromosomes of the silkworms, Bombyx mori and B. mandarina, (Bombycidae, Lepidoptera). Chromosoma. 1974;47(2):167–178. doi: 10.1007/BF00331804. [DOI] [PubMed] [Google Scholar]
- Puck T. T., Marcus P. I. A RAPID METHOD FOR VIABLE CELL TITRATION AND CLONE PRODUCTION WITH HELA CELLS IN TISSUE CULTURE: THE USE OF X-IRRADIATED CELLS TO SUPPLY CONDITIONING FACTORS. Proc Natl Acad Sci U S A. 1955 Jul 15;41(7):432–437. doi: 10.1073/pnas.41.7.432. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Trosko J. E., Wilder K. Repair of UV-induced pyrimidine dimers in Drosophila melanogaster cells in vitro. Genetics. 1973 Feb;73(2):297–302. doi: 10.1093/genetics/73.2.297. [DOI] [PMC free article] [PubMed] [Google Scholar]