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. 1983 Aug;80(16):4879–4883. doi: 10.1073/pnas.80.16.4879

A previously unrecognized influenza B virus glycoprotein from a bicistronic mRNA that also encodes the viral neuraminidase.

M W Shaw, P W Choppin, R A Lamb
PMCID: PMC384150  PMID: 6308656

Abstract

RNA segment 6 of the influenza B virus genome codes for a previously unidentified polypeptide designated NB. The reading frame for this polypeptide begins with the first AUG codon on the mRNA and overlaps the reading frame for the viral neuraminidase by 292 nucleotides. The amino acid sequence of polypeptide NB deduced from the nucleotide sequence of the B/Lee/40 strain consists of 100 amino acids with a molecular weight of 11,242. The sequence contains four potential glycosylation sites, and the protein has been found to be glycosylated in infected cells. NB has not been found in virions. Sucrose gradient sedimentation and analysis of the structure of the mRNA by nuclease S1 mapping and sequence analysis by the primer extension method indicated that polypeptide NB and the neuraminidase are translated from a single bicistronic mRNA. A protein analogous to NB has not been found with influenza A virus, and this represents a major difference between the two virus types.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Akashi H., Bishop D. H. Comparison of the sequences and coding of La Crosse and snowshoe hare bunyavirus S RNA species. J Virol. 1983 Mar;45(3):1155–1158. doi: 10.1128/jvi.45.3.1155-1158.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Blok J., Air G. M. Sequence variation at the 3' end of the neuraminidase gene from 39 influenza type A viruses. Virology. 1982 Sep;121(2):211–229. doi: 10.1016/0042-6822(82)90162-3. [DOI] [PubMed] [Google Scholar]
  3. Bos J. L., Polder L. J., Bernards R., Schrier P. I., van den Elsen P. J., van der Eb A. J., van Ormondt H. The 2.2 kb E1b mRNA of human Ad12 and Ad5 codes for two tumor antigens starting at different AUG triplets. Cell. 1981 Nov;27(1 Pt 2):121–131. doi: 10.1016/0092-8674(81)90366-4. [DOI] [PubMed] [Google Scholar]
  4. Briedis D. J., Lamb R. A., Choppin P. W. Influenza B virus RNA segment 8 codes for two nonstructural proteins. Virology. 1981 Jul 30;112(2):417–425. doi: 10.1016/0042-6822(81)90289-0. [DOI] [PubMed] [Google Scholar]
  5. Briedis D. J., Lamb R. A., Choppin P. W. Sequence of RNA segment 7 of the influenza B virus genome: partial amino acid homology between the membrane proteins (M1) of influenza A and B viruses and conservation of a second open reading frame. Virology. 1982 Jan 30;116(2):581–588. doi: 10.1016/0042-6822(82)90150-7. [DOI] [PubMed] [Google Scholar]
  6. Briedis D. J., Lamb R. A. Influenza B virus genome: sequences and structural organization of RNA segment 8 and the mRNAs coding for the NS1 and NS2 proteins. J Virol. 1982 Apr;42(1):186–193. doi: 10.1128/jvi.42.1.186-193.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Collins P. L., Hightower L. E., Ball L. A. Transcription and translation of Newcastle disease virus mRNA's in vitro. J Virol. 1978 Oct;28(1):324–336. doi: 10.1128/jvi.28.1.324-336.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Erickson A. H., Conner G. E., Blobel G. Biosynthesis of a lysosomal enzyme. Partial structure of two transient and functionally distinct NH2-terminal sequences in cathepsin D. J Biol Chem. 1981 Nov 10;256(21):11224–11231. [PubMed] [Google Scholar]
  9. Fields S., Winter G., Brownlee G. G. Structure of the neuraminidase gene in human influenza virus A/PR/8/34. Nature. 1981 Mar 19;290(5803):213–217. doi: 10.1038/290213a0. [DOI] [PubMed] [Google Scholar]
  10. Gruss P., Efstratiadis A., Karathanasis S., König M., Khoury G. Synthesis of stable unspliced mRNA from an intronless simian virus 40--rat preproinsulin gene recombinant. Proc Natl Acad Sci U S A. 1981 Oct;78(10):6091–6095. doi: 10.1073/pnas.78.10.6091. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Hiti A. L., Nayak D. P. Complete nucleotide sequence of the neuraminidase gene of human influenza virus A/WSN/33. J Virol. 1982 Feb;41(2):730–734. doi: 10.1128/jvi.41.2.730-734.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Jay G., Nomura S., Anderson C. W., Khoury G. Identification of the SV40 agnogene product: a DNA binding protein. Nature. 1981 May 28;291(5813):346–349. doi: 10.1038/291346a0. [DOI] [PubMed] [Google Scholar]
  13. Kozak M. How do eucaryotic ribosomes select initiation regions in messenger RNA? Cell. 1978 Dec;15(4):1109–1123. doi: 10.1016/0092-8674(78)90039-9. [DOI] [PubMed] [Google Scholar]
  14. Kozak M. Mechanism of mRNA recognition by eukaryotic ribosomes during initiation of protein synthesis. Curr Top Microbiol Immunol. 1981;93:81–123. doi: 10.1007/978-3-642-68123-3_5. [DOI] [PubMed] [Google Scholar]
  15. Krystal M., Elliott R. M., Benz E. W., Jr, Young J. F., Palese P. Evolution of influenza A and B viruses: conservation of structural features in the hemagglutinin genes. Proc Natl Acad Sci U S A. 1982 Aug;79(15):4800–4804. doi: 10.1073/pnas.79.15.4800. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Lamb R. A., Choppin P. W., Chanock R. M., Lai C. J. Mapping of the two overlapping genes for polypeptides NS1 and NS2 on RNA segment 8 of influenza virus genome. Proc Natl Acad Sci U S A. 1980 Apr;77(4):1857–1861. doi: 10.1073/pnas.77.4.1857. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Lamb R. A., Choppin P. W. Segment 8 of the influenza virus genome is unique in coding for two polypeptides. Proc Natl Acad Sci U S A. 1979 Oct;76(10):4908–4912. doi: 10.1073/pnas.76.10.4908. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Lamb R. A., Etkind P. R., Choppin P. W. Evidence for a ninth influenza viral polypeptide. Virology. 1978 Nov;91(1):60–78. doi: 10.1016/0042-6822(78)90355-0. [DOI] [PubMed] [Google Scholar]
  19. Lamb R. A., Lai C. J., Choppin P. W. Sequences of mRNAs derived from genome RNA segment 7 of influenza virus: colinear and interrupted mRNAs code for overlapping proteins. Proc Natl Acad Sci U S A. 1981 Jul;78(7):4170–4174. doi: 10.1073/pnas.78.7.4170. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Lamb R. A., Lai C. J. Sequence of interrupted and uninterrupted mRNAs and cloned DNA coding for the two overlapping nonstructural proteins of influenza virus. Cell. 1980 Sep;21(2):475–485. doi: 10.1016/0092-8674(80)90484-5. [DOI] [PubMed] [Google Scholar]
  21. Lamb R. A., Lai C. J. Spliced and unspliced messenger RNAs synthesized from cloned influenza virus M DNA in an SV40 vector: expression of the influenza virus membrane protein (M1). Virology. 1982 Dec;123(2):237–256. doi: 10.1016/0042-6822(82)90258-6. [DOI] [PubMed] [Google Scholar]
  22. Markoff L., Lai C. J. Sequence of the influenza A/Udorn/72 (H3N2) virus neuraminidase gene as determined from cloned full-length DNA. Virology. 1982 Jun;119(2):288–297. doi: 10.1016/0042-6822(82)90089-7. [DOI] [PubMed] [Google Scholar]
  23. Mertens P. P., Dobos P. Messenger RNA of infectious pancreatic necrosis virus is polycistronic. Nature. 1982 May 20;297(5863):243–246. doi: 10.1038/297243a0. [DOI] [PubMed] [Google Scholar]
  24. Mertz J. E., Murphy A., Barkan A. Mutants deleted in the agnogene of simian virus 40 define a new complementation group. J Virol. 1983 Jan;45(1):36–46. doi: 10.1128/jvi.45.1.36-46.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Palese P., Schulman J. L. Mapping of the influenza virus genome: identification of the hemagglutinin and the neuraminidase genes. Proc Natl Acad Sci U S A. 1976 Jun;73(6):2142–2146. doi: 10.1073/pnas.73.6.2142. [DOI] [PMC free article] [PubMed] [Google Scholar]
  26. Racaniello V. R., Palese P. Influenza B virus genome: assignment of viral polypeptides to RNA segments. J Virol. 1979 Jan;29(1):361–373. doi: 10.1128/jvi.29.1.361-373.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  27. Shaw M. W., Lamb R. A., Erickson B. W., Briedis D. J., Choppin P. W. Complete nucleotide sequence of the neuraminidase gene of influenza B virus. Proc Natl Acad Sci U S A. 1982 Nov;79(22):6817–6821. doi: 10.1073/pnas.79.22.6817. [DOI] [PMC free article] [PubMed] [Google Scholar]
  28. Ueda M., Tobita K., Sugiura A., Enomoto C. Identification of hemagglutinin and neuraminidase genes of influenza B virus. J Virol. 1978 Feb;25(2):685–686. doi: 10.1128/jvi.25.2.685-686.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]

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