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. 1983 Aug;80(16):4904–4908. doi: 10.1073/pnas.80.16.4904

Molecular cloning and sequence of partial cDNA for interferon-induced (2'-5')oligo(A) synthetase mRNA from human cells.

G Merlin, J Chebath, P Benech, R Metz, M Revel
PMCID: PMC384155  PMID: 6348777

Abstract

By using a translation assay in oocytes, a 17S RNA fraction coding for the interferon-induced (2'-5')oligo(A) synthetase was purified from human cells. A cDNA library was prepared by cloning in Escherichia coli plasmid pBR322 and screened by positive hybridization-translation in oocytes. A cDNA clone corresponding to the (2'-5')oligo(A) synthetase mRNA was identified. In SV80 cells, this E cDNA recognizes three RNAs of 1.65, 1.85, and 3.6 kilobases, which are present only after interferon treatment of the cells. In Namalva cells, mainly one RNA of 1.8 kilobases is seen.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Backman K., Ptashne M., Gilbert W. Construction of plasmids carrying the cI gene of bacteriophage lambda. Proc Natl Acad Sci U S A. 1976 Nov;73(11):4174–4178. doi: 10.1073/pnas.73.11.4174. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Baglioni C. Interferon-induced enzymatic activities and their role in the antriviral state. Cell. 1979 Jun;17(2):255–264. doi: 10.1016/0092-8674(79)90151-x. [DOI] [PubMed] [Google Scholar]
  3. Baglioni C., Minks M. A., Maroney P. A. Interferon action may be mediated by activation of a nuclease by pppA2'p5'A2'p5'A. Nature. 1978 Jun 22;273(5664):684–687. doi: 10.1038/273684a0. [DOI] [PubMed] [Google Scholar]
  4. Berk A. J., Sharp P. A. Sizing and mapping of early adenovirus mRNAs by gel electrophoresis of S1 endonuclease-digested hybrids. Cell. 1977 Nov;12(3):721–732. doi: 10.1016/0092-8674(77)90272-0. [DOI] [PubMed] [Google Scholar]
  5. Chebath J., Merlin G., Metz R., Benech P., Revel M. Interferon-induced 56,000 Mr protein and its mRNA in human cells: molecular cloning and partial sequence of the cDNA. Nucleic Acids Res. 1983 Mar 11;11(5):1213–1226. doi: 10.1093/nar/11.5.1213. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Clewell D. B., Helinski D. R. Supercoiled circular DNA-protein complex in Escherichia coli: purification and induced conversion to an opern circular DNA form. Proc Natl Acad Sci U S A. 1969 Apr;62(4):1159–1166. doi: 10.1073/pnas.62.4.1159. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Epstein D. A., Czarniecki C. W., Jacobsen H., Friedman R. M., Panet A. A mouse cell line, which is unprotected by interferon against lytic virus infection, lacks ribonuclease F activity. Eur J Biochem. 1981 Aug;118(1):9–15. doi: 10.1111/j.1432-1033.1981.tb05479.x. [DOI] [PubMed] [Google Scholar]
  8. Fellous M., Kamoun M., Gresser I., Bono R. Enhanced expression of HLA antigens and beta 2-microglobulin on interferon-treated human lymphoid cells. Eur J Immunol. 1979 Jun;9(6):446–449. doi: 10.1002/eji.1830090606. [DOI] [PubMed] [Google Scholar]
  9. Fellous M., Nir U., Wallach D., Merlin G., Rubinstein M., Revel M. Interferon-dependent induction of mRNA for the major histocompatibility antigens in human fibroblasts and lymphoblastoid cells. Proc Natl Acad Sci U S A. 1982 May;79(10):3082–3086. doi: 10.1073/pnas.79.10.3082. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Gurdon J. B., Lane C. D., Woodland H. R., Marbaix G. Use of frog eggs and oocytes for the study of messenger RNA and its translation in living cells. Nature. 1971 Sep 17;233(5316):177–182. doi: 10.1038/233177a0. [DOI] [PubMed] [Google Scholar]
  11. Heron I., Hokland M., Berg K. Enhanced expression of beta2-microglobulin and HLA antigens on human lymphoid cells by interferon. Proc Natl Acad Sci U S A. 1978 Dec;75(12):6215–6219. doi: 10.1073/pnas.75.12.6215. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Kerr I. M., Brown R. E. pppA2'p5'A2'p5'A: an inhibitor of protein synthesis synthesized with an enzyme fraction from interferon-treated cells. Proc Natl Acad Sci U S A. 1978 Jan;75(1):256–260. doi: 10.1073/pnas.75.1.256. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Kimchi A. Increased levels of interferon-induced (2'--5') oligo-isoadenylate synthetase in mature T-lymphocytes and in differentiated Friend-erythroleukemic cells. J Interferon Res. 1981;1(4):559–569. doi: 10.1089/jir.1981.1.559. [DOI] [PubMed] [Google Scholar]
  14. Kimchi A., Shure H., Lapidot Y., Rapoport S., Panet A., Revel M. Antimitogenic effects of interferon and (2'-5')-oligoadenylate in synchronized 3T3 fibroblasts. FEBS Lett. 1981 Nov 16;134(2):212–216. doi: 10.1016/0014-5793(81)80604-7. [DOI] [PubMed] [Google Scholar]
  15. Kimchi A., Shure H., Revel M. Anti-mitogenic function of interferon-induced (2'-5')oligo(adenylate) and growth-related variations in enzymes that synthesize and degrade this oligonucleotide. Eur J Biochem. 1981;114(1):5–10. doi: 10.1111/j.1432-1033.1981.tb06163.x. [DOI] [PubMed] [Google Scholar]
  16. Knight E., Jr, Fahey D. Human fibroblast interferon. An improved purification. J Biol Chem. 1981 Apr 25;256(8):3609–3611. [PubMed] [Google Scholar]
  17. Knight E., Jr, Korant B. D. Fibroblast interferon induces synthesis of four proteins in human fibroblast cells. Proc Natl Acad Sci U S A. 1979 Apr;76(4):1824–1827. doi: 10.1073/pnas.76.4.1824. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Krishnan I., Baglioni C. Increased levels of (2'-5')oligo(A) polymerase activity in human lymphoblastoid cells treated with glucocorticoids. Proc Natl Acad Sci U S A. 1980 Nov;77(11):6506–6510. doi: 10.1073/pnas.77.11.6506. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Land H., Grez M., Hauser H., Lindenmaier W., Schütz G. 5'-Terminal sequences of eucaryotic mRNA can be cloned with high efficiency. Nucleic Acids Res. 1981 May 25;9(10):2251–2266. doi: 10.1093/nar/9.10.2251. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. LeMeur M., Glanville N., Mandel J. L., Gerlinger P., Palmiter R., Chambon P. The ovalbumin gene family: hormonal control of X and Y gene transcription and mRNA accumulation. Cell. 1981 Feb;23(2):561–571. doi: 10.1016/0092-8674(81)90152-5. [DOI] [PubMed] [Google Scholar]
  21. Lebleu B., Content J. Mechanisms of interferon action: biochemical and genetic approaches. Interferon. 1982;4:47–94. [PubMed] [Google Scholar]
  22. Lengyel P. Biochemistry of interferons and their actions. Annu Rev Biochem. 1982;51:251–282. doi: 10.1146/annurev.bi.51.070182.001343. [DOI] [PubMed] [Google Scholar]
  23. Maxam A. M., Gilbert W. Sequencing end-labeled DNA with base-specific chemical cleavages. Methods Enzymol. 1980;65(1):499–560. doi: 10.1016/s0076-6879(80)65059-9. [DOI] [PubMed] [Google Scholar]
  24. Mory Y., Chernajovsky Y., Feinstein S. I., Chen L., Nir U., Weissenbach J., Malpiece Y., Tiollais P., Marks D., Ladner M. Synthesis of human interferon beta 1 in Escherichia coli infected by a lambda phage recombinant containing a human genomic fragment. Eur J Biochem. 1981 Nov;120(1):197–202. doi: 10.1111/j.1432-1033.1981.tb05689.x. [DOI] [PubMed] [Google Scholar]
  25. Nagata S., Taira H., Hall A., Johnsrud L., Streuli M., Ecsödi J., Boll W., Cantell K., Weissmann C. Synthesis in E. coli of a polypeptide with human leukocyte interferon activity. Nature. 1980 Mar 27;284(5754):316–320. doi: 10.1038/284316a0. [DOI] [PubMed] [Google Scholar]
  26. Novick D., Eshhar Z., Gigi O., Marks Z., Revel M., Rubinstein M. Affinity chromatography of human fibroblast interferon (IFN-beta 1) by monoclonal antibody columns. J Gen Virol. 1983 Apr;64(Pt 4):905–910. doi: 10.1099/0022-1317-64-4-905. [DOI] [PubMed] [Google Scholar]
  27. Novick D., Eshhar Z., Rubinstein M. Monoclonal antibodies to human alpha-interferon and their use for affinity chromatography. J Immunol. 1982 Nov;129(5):2244–2247. [PubMed] [Google Scholar]
  28. Proudfoot N. J., Brownlee G. G. 3' non-coding region sequences in eukaryotic messenger RNA. Nature. 1976 Sep 16;263(5574):211–214. doi: 10.1038/263211a0. [DOI] [PubMed] [Google Scholar]
  29. Revel M. F., Kimchi A. Initial characterization of a spontaneous interferon secreted during growth and differentiation of Friend erythroleukemia cells. Mol Cell Biol. 1982 Dec;2(12):1472–1480. doi: 10.1128/mcb.2.12.1472. [DOI] [PMC free article] [PubMed] [Google Scholar]
  30. Revel M., Kimchi A., Friedman M., Wolf D., Merlin G., Panet A., Rapoport S., Lapidot Y. Cell-regulatory functions of interferon induced enzymes: antimitogenic effect of (2'-5')oligo-A, growth-related variations in (2'-5')oligo-A synthetase, and isolation of its mRNA. Tex Rep Biol Med. 1981;41:452–462. [PubMed] [Google Scholar]
  31. Revel M., Wallach D., Merlin G., Schattner A., Schmidt A., Wolf D., Shulman L., Kimchi A. Interferon-induced enzymes: microassays and their applications: purification and assay of (2'-5')-oligoadenylate synthetase and assay of 2'-phosphodiesterase. Methods Enzymol. 1981;79(Pt B):149–161. doi: 10.1016/s0076-6879(81)79024-4. [DOI] [PubMed] [Google Scholar]
  32. Rosen J. M., Woo S. L., Holder J. W., Means A. R., O'Malley B. W. Preparation and preliminary characterization of purified ovalbumin messenger RNA from the hen oviduct. Biochemistry. 1975 Jan 14;14(1):69–78. doi: 10.1021/bi00672a012. [DOI] [PubMed] [Google Scholar]
  33. Rubin B. Y., Gupta S. L. Interferon-induced proteins in human fibroblasts and development of the antiviral state. J Virol. 1980 May;34(2):446–454. doi: 10.1128/jvi.34.2.446-454.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  34. Schattner A., Merlin G., Wallach D., Rosenberg H., Bino T., Hahn T., Levin S., Revel M. Monitoring of interferon therapy by assay of (2'--5') oligo-isoadenylate synthetase in human peripheral white blood cells. J Interferon Res. 1981;1(4):587–594. doi: 10.1089/jir.1981.1.587. [DOI] [PubMed] [Google Scholar]
  35. Schattner A., Wallach D., Merlin G., Hahn T., Levin S., Ramot B., Revel M. Variation of (2'-5') oligo A synthetase level in lymphocytes and granulocytes of patients with viral infections and leukemia. J Interferon Res. 1982;2(3):355–361. doi: 10.1089/jir.1982.2.355. [DOI] [PubMed] [Google Scholar]
  36. Schattner A., Wallach D., Merlin G., Hahn T., Levin S., Revel M. Assay of an interferon-induced enzyme in white blood cells as a diagnostic aid in viral diseases. Lancet. 1981 Sep 5;2(8245):497–500. doi: 10.1016/s0140-6736(81)90883-7. [DOI] [PubMed] [Google Scholar]
  37. Schmidt A., Zilberstein A., Shulman L., Federman P., Berissi H., Revel M. Interferon action: isolation of nuclease F, a translation inhibitor activated by interferon-induced (2'-5') oligo-isoadenylate. FEBS Lett. 1978 Nov 15;95(2):257–264. doi: 10.1016/0014-5793(78)81006-0. [DOI] [PubMed] [Google Scholar]
  38. Setzer D. R., McGrogan M., Schimke R. T. Nucleotide sequence surrounding multiple polyadenylation sites in the mouse dihydrofolate reductase gene. J Biol Chem. 1982 May 10;257(9):5143–5147. [PubMed] [Google Scholar]
  39. Shulman L., Revel M. Interferon-dependent induction of mRNA activity for (2'-5')oligo-isoadenylate synthetase. Nature. 1980 Nov 6;288(5786):98–100. doi: 10.1038/288098a0. [DOI] [PubMed] [Google Scholar]
  40. Slattery E., Ghosh N., Samanta H., Lengyel P. Interferon, double-stranded RNA, and RNA degradation: activation of an endonuclease by (2'-5')An. Proc Natl Acad Sci U S A. 1979 Oct;76(10):4778–4782. doi: 10.1073/pnas.76.10.4778. [DOI] [PMC free article] [PubMed] [Google Scholar]
  41. Stark G. R., Dower W. J., Schimke R. T., Brown R. E., Kerr I. M. 2-5A synthetase: assay, distribution and variation with growth or hormone status. Nature. 1979 Mar 29;278(5703):471–473. doi: 10.1038/278471a0. [DOI] [PubMed] [Google Scholar]
  42. Weissenbach J., Zeevi M., Landau T., Revel M. Identification of the translation products of human fibroblast interferon mRNA in reticulocyte lysates. Eur J Biochem. 1979 Jul;98(1):1–8. doi: 10.1111/j.1432-1033.1979.tb13153.x. [DOI] [PubMed] [Google Scholar]
  43. Wickens M. P., Buell G. N., Schimke R. T. Synthesis of double-stranded DNA complementary to lysozyme, ovomucoid, and ovalbumin mRNAs. Optimization for full length second strand synthesis by Escherichia coli DNA polymerase I. J Biol Chem. 1978 Apr 10;253(7):2483–2495. [PubMed] [Google Scholar]
  44. Yang K., Samanta H., Dougherty J., Jayaram B., Broeze R., Lengyel P. Interferons, double-stranded RNA, and RNA degradation. Isolation and characterization of homogeneous human (2'-5')(a)n synthetase. J Biol Chem. 1981 Sep 10;256(17):9324–9328. [PubMed] [Google Scholar]

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