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. 1983 Aug;80(16):4922–4925. doi: 10.1073/pnas.80.16.4922

12-fold difference between the critical monomer concentrations of the two ends of actin filaments in physiological salt conditions.

A Wegner, G Isenberg
PMCID: PMC384159  PMID: 6576365

Abstract

We determined the critical monomer concentrations at which association and dissociation reactions are balanced at the two ends of actin filaments. For measurement of the critical concentration of the pointed end, interference with the high dynamics of the barbed end was excluded by capping the barbed ends with an actin filament capping protein isolated from bovine brain. The critical concentration of the pointed end (1.5 microM) was found to be 12- to 15-fold higher than the critical concentration of the barbed end (0.10-0.12 microM) at a temperature of 37 degrees C and physiological salt concentrations (100 mM KCl/1-2 mM MgCl2/0.3 mM EGTA or 0.2 mM CaCl2, pH 7.5).

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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