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. 1983 Aug;80(16):4940–4944. doi: 10.1073/pnas.80.16.4940

Characterization and nucleotide sequence of a chicken gene encoding an opal suppressor tRNA and its flanking DNA segments.

D L Hatfield, B S Dudock, F C Eden
PMCID: PMC384163  PMID: 6308662

Abstract

A naturally occurring opal suppressor serine tRNA has been purified from chicken liver and used as a probe to isolate the corresponding gene from a library of chicken DNA in bacteriophage lambda. This minor tRNA is encoded by a single-copy gene that is not part of a tRNA gene cluster. DNA sequence analysis of the gene and its flanking DNA segments shows that the gene is encoded in an 87-base-pair segment without intervening sequences and specifies a tRNA that reads the termination codon UGA. This gene has additional nucleotides in the 5' internal promoter region but has a normal 3' internal promoter sequence and the usual termination signal.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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