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. 1983 Aug;80(16):4945–4948. doi: 10.1073/pnas.80.16.4945

High-resolution 1H nuclear magnetic resonance study of cerebral hypoxia in vivo.

K L Behar, J A den Hollander, M E Stromski, T Ogino, R G Shulman, O A Petroff, J W Prichard
PMCID: PMC384164  PMID: 6576367

Abstract

1H NMR spectra at 360.13 MHz were obtained of the rat brain in vivo by using a surface coil placed over the skull. Resonances of numerous metabolites were identified by comparison with the 1H NMR spectra of excised rat brain tissue and acid extracts of the tissue. Changes in cerebral lactate levels resulting from the administration of gas mixtures low in oxygen were monitored in the in vivo brain spectra with a time resolution of 2.3 min. The electroencephalogram and electrocardiogram were recorded simultaneously during the NMR experiment. Reversibility of the hypoxic response was documented when, upon oxygen administration, cerebral lactate returned to its prehypoxic level. These experiments demonstrate the applicability of high-resolution 1H NMR to monitor pathophysiology of brain metabolism in real time.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Ackerman J. J., Grove T. H., Wong G. G., Gadian D. G., Radda G. K. Mapping of metabolites in whole animals by 31P NMR using surface coils. Nature. 1980 Jan 10;283(5743):167–170. doi: 10.1038/283167a0. [DOI] [PubMed] [Google Scholar]
  2. Agris P. F., Campbell I. D. Proton nuclear magnetic resonance of intact Friend leukemia cells: phosphorylcholine increase during differentiation. Science. 1982 Jun 18;216(4552):1325–1327. doi: 10.1126/science.7079765. [DOI] [PubMed] [Google Scholar]
  3. Alger J. R., Sillerud L. O., Behar K. L., Gillies R. J., Shulman R. G., Gordon R. E., Shae D., Hanley P. E. In vivo carbon-13 nuclear magnetic resonance studies of mammals. Science. 1981 Nov 6;214(4521):660–662. doi: 10.1126/science.7292005. [DOI] [PubMed] [Google Scholar]
  4. Brindle K. M., Brown F. F., Campbell I. D., Grathwohl C., Kuchel P. W. Application of spin-echo nuclear magnetic resonance to whole-cell systems. Membrane transport. Biochem J. 1979 Apr 15;180(1):37–44. doi: 10.1042/bj1800037. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Brown F. F., Campbell I. D., Kuchel P. W., Rabenstein D. C. Human erythrocyte metabolism studies by 1H spin echo NMR. FEBS Lett. 1977 Oct 1;82(1):12–16. doi: 10.1016/0014-5793(77)80875-2. [DOI] [PubMed] [Google Scholar]
  6. Buonanno F. S., Pykett I. L., Kistler J. P., Vielma J., Brady T. J., Hinshaw W. S., Goldman M. R., Newhouse J. H., Pohost G. M. Cranial anatomy and detection of ischemic stroke in the cat by nuclear magnetic resonance imaging. Radiology. 1982 Apr;143(1):187–193. doi: 10.1148/radiology.143.1.7063725. [DOI] [PubMed] [Google Scholar]
  7. Campbell I. D., Dobson C. M., Jeminet G., Williams R. J. Pulsed NMR methods for the observation and assignment of exchangeable hydrogens: application to bacitracin. FEBS Lett. 1974 Dec 1;49(1):115–119. doi: 10.1016/0014-5793(74)80645-9. [DOI] [PubMed] [Google Scholar]
  8. Chance B., Nakase Y., Bond M., Leigh J. S., Jr, McDonald G. Detection of 31P nuclear magnetic resonance signals in brain by in vivo and freeze-trapped assays. Proc Natl Acad Sci U S A. 1978 Oct;75(10):4925–4929. doi: 10.1073/pnas.75.10.4925. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Daniels A., Williams R. J., Wright P. E. Nuclear magnetic resonance studies of the adrenal gland and some other organs. Nature. 1976 May 27;261(5558):321–323. doi: 10.1038/261321a0. [DOI] [PubMed] [Google Scholar]
  10. Edwards R. H., Dawson M. J., Wilkie D. R., Gordon R. E., Shaw D. Clinical use of nuclear magnetic resonance in the investigation of myopathy. Lancet. 1982 Mar 27;1(8274):725–731. doi: 10.1016/s0140-6736(82)92635-6. [DOI] [PubMed] [Google Scholar]
  11. Folbergrová J., MacMillan V., Siesjö B. K. The effect of moderate and marked hypercapnia upon the energy state and upon the cytoplasmic NADH-NAD+ ratio of the rat brain. J Neurochem. 1972 Nov;19(11):2497–2505. doi: 10.1111/j.1471-4159.1972.tb01309.x. [DOI] [PubMed] [Google Scholar]
  12. Ljunggren B., Norberg K., Siesjö B. K. Influence of tissue acidosis upon restitution of brain energy metabolism following total ischemia. Brain Res. 1974 Sep 6;77(2):173–186. doi: 10.1016/0006-8993(74)90782-3. [DOI] [PubMed] [Google Scholar]
  13. Neurohr K. J., Barrett E. J., Shulman R. G. In vivo carbon-13 nuclear magnetic resonance studies of heart metabolism. Proc Natl Acad Sci U S A. 1983 Mar;80(6):1603–1607. doi: 10.1073/pnas.80.6.1603. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Norberg K., Siesjö B. K. Cerebral metabolism in hypoxic hypoxia. I. Pattern of activation of glycolysis: a re-evaluation. Brain Res. 1975 Mar 14;86(1):31–44. doi: 10.1016/0006-8993(75)90635-6. [DOI] [PubMed] [Google Scholar]
  15. Ogino T., Arata Y., Fujiwara S. Proton correlation nuclear magnetic resonance study of metabolic regulations and pyruvate transport in anaerobic Escherichia coli cells. Biochemistry. 1980 Aug 5;19(16):3684–3691. doi: 10.1021/bi00557a008. [DOI] [PubMed] [Google Scholar]
  16. Pontén U., Ratcheson R. A., Salford L. G., Siesjö B. K. Optimal freezing conditions for cerebral metabolites in rats. J Neurochem. 1973 Nov;21(5):1127–1138. doi: 10.1111/j.1471-4159.1973.tb07567.x. [DOI] [PubMed] [Google Scholar]
  17. Prichard J. W., Alger J. R., Behar K. L., Petroff O. A., Shulman R. G. Cerebral metabolic studies in vivo by 31P NMR. Proc Natl Acad Sci U S A. 1983 May;80(9):2748–2751. doi: 10.1073/pnas.80.9.2748. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Rehncrona S., Rosén I., Siesjö B. K. Excessive cellular acidosis: an important mechanism of neuronal damage in the brain? Acta Physiol Scand. 1980 Dec;110(4):435–437. doi: 10.1111/j.1748-1716.1980.tb06692.x. [DOI] [PubMed] [Google Scholar]
  19. Ross B. D., Radda G. K., Gadian D. G., Rocker G., Esiri M., Falconer-Smith J. Examination of a case of suspected McArdle's syndrome by 31P nuclear magnetic resonance. N Engl J Med. 1981 May 28;304(22):1338–1342. doi: 10.1056/NEJM198105283042206. [DOI] [PubMed] [Google Scholar]
  20. Shulman R. G., Brown T. R., Ugurbil K., Ogawa S., Cohen S. M., den Hollander J. A. Cellular applications of 31P and 13C nuclear magnetic resonance. Science. 1979 Jul 13;205(4402):160–166. doi: 10.1126/science.36664. [DOI] [PubMed] [Google Scholar]
  21. Sillerud L. O., Shulman R. G. Structure and metabolism of mammalian liver glycogen monitored by carbon-13 nuclear magnetic resonance. Biochemistry. 1983 Mar 1;22(5):1087–1094. doi: 10.1021/bi00274a015. [DOI] [PubMed] [Google Scholar]
  22. Thulborn K. R., du Boulay G. H., Duchen L. W., Radda G. A 31P nuclear magnetic resonance in vivo study of cerebral ischaemia in the gerbil. J Cereb Blood Flow Metab. 1982 Sep;2(3):299–306. doi: 10.1038/jcbfm.1982.31. [DOI] [PubMed] [Google Scholar]
  23. Walker T. E., Han C. H., Kollman V. H., London R. E., Matwiyoff N. A. 13C nuclear magnetic resonance studies of the biosynthesis by Microbacterium ammoniaphilum of L-glutamate selectively enriched with carbon-13. J Biol Chem. 1982 Feb 10;257(3):1189–1195. [PubMed] [Google Scholar]

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