Skip to main content
NCBI home page
Proceedings of the National Academy of Sciences of the United States of America logoLink to Proceedings of the National Academy of Sciences of the United States of America
. 1983 Aug;80(16):5027–5031. doi: 10.1073/pnas.80.16.5027

Two nonallelic tRNAiMet genes are located in the p23 leads to q12 region of human chromosome 6.

S L Naylor, A Y Sakaguchi, T B Shows, K H Grzeschik, M Holmes, M Zasloff
PMCID: PMC384181  PMID: 6308668

Abstract

Two nonallelic human tRNAiMet genes were assigned to chromosome 6 by filter hybridization of DNA from human-rodent somatic cell hybrids by using probes containing unique sequences from the regions flanking each tRNAiMet gene. These unique sequence probes thus allowed each tRNAiMet gene to be analyzed individually in cell hybrids. Both tRNAiMet genes segregated in the hybrid cells with the chromosome 6 enzyme markers, soluble malic enzyme and the mitochondrial form of superoxide dismutase, and also with a karyotypically normal chromosome 6. By using hybrid clones containing translocations that divide chromosome 6 into five segments, both tRNAiMet genes were assigned to the p23 leads to q12 region. These results raise the possibility that other tRNAiMet genes may be syntenic with the two described in this study and illustrate the utility of using unique flanking sequences to identify members of a multigene family.

Full text

PDF
5027

Images in this article

5028Image
on p.5028
5028Image
on p.5028

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Champion M. J., Brown J. A., Shows T. B. Assignment of cytoplasmic alpha-mannosidase (MANA) and confirmation of mitochondrial isocitrate dehydrogenase (IDHM) to the q11 leads to qter region of chromosome 15 in man. Cytogenet Cell Genet. 1978;22(1-6):498–502. doi: 10.1159/000131007. [DOI] [PubMed] [Google Scholar]
  2. Champion M. J., Shows T. B. Mannosidosis: assignment of the lysosomal alpha-mannosidase B gene to chromosome 19 in man. Proc Natl Acad Sci U S A. 1977 Jul;74(7):2968–2972. doi: 10.1073/pnas.74.7.2968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Chandler M. E., Kedes L. H., Cohn R. H., Yunis J. J. Genes coding for histone proteins in man are located on the distal end of the long arm of chromosome 7. Science. 1979 Aug 31;205(4409):908–910. doi: 10.1126/science.472711. [DOI] [PubMed] [Google Scholar]
  4. Chen T. R., McMorris F. A., Creagan R., Ricciuti F., Tischfield J., Ruddle F. Assignment of the genes for malate oxidoreductase decarboxylating to chromosome 6 and peptidase B and lactate dehydrogenase B to chromosome 12 in man. Am J Hum Genet. 1973 Mar;25(2):200–207. [PMC free article] [PubMed] [Google Scholar]
  5. Clarkson S. G., Kurer V. Isolation and some properties of DNA coding for tRNA1met from Xenopus laevis. Cell. 1976 Jun;8(2):183–195. doi: 10.1016/0092-8674(76)90002-7. [DOI] [PubMed] [Google Scholar]
  6. Clarkson S. G., Kurer V., Smith H. O. Sequence organization of a cloned tDNA met fragment from Xenopus laevis. Cell. 1978 Jul;14(3):713–724. doi: 10.1016/0092-8674(78)90253-2. [DOI] [PubMed] [Google Scholar]
  7. Creagan R., Tischfield J., Ricciuti F., Ruddle F. H. Chromosome assignments of genes in man using mouse-human somatic cell hybrids: mitochondrial superoxide dismutase (indophenol oxidase-B, tetrameric) to chromosome 6. Humangenetik. 1973 Dec 10;20(3):203–209. doi: 10.1007/BF00385731. [DOI] [PubMed] [Google Scholar]
  8. Davidson R. L., Gerald P. S. Improved techniques for the induction of mammalian cell hybridization by polyethylene glycol. Somatic Cell Genet. 1976 Mar;2(2):165–176. doi: 10.1007/BF01542629. [DOI] [PubMed] [Google Scholar]
  9. Deisseroth A., Nienhuis A., Turner P., Velez R., Anderson W. F., Ruddle F., Lawrence J., Creagan R., Kucherlapati R. Localization of the human alpha-globin structural gene to chromosome 16 in somatic cell hybrids by molecular hybridization assay. Cell. 1977 Sep;12(1):205–218. doi: 10.1016/0092-8674(77)90198-2. [DOI] [PubMed] [Google Scholar]
  10. Fedoroff N. V. On spacers. Cell. 1979 Apr;16(4):697–710. doi: 10.1016/0092-8674(79)90086-2. [DOI] [PubMed] [Google Scholar]
  11. Grzeschik K. H. Assignment of a structural gene for beta-glucuronidase to human chromosome C7. Somatic Cell Genet. 1976 Sep;2(5):401–410. doi: 10.1007/BF01542721. [DOI] [PubMed] [Google Scholar]
  12. Grzeschik K. H., Mayerová A., Bender K., Johannsmann R. Regional submapping of human chromosome 6 by the use of human-mouse somatic cell hybrids. Cytogenet Cell Genet. 1978;22(1-6):412–414. doi: 10.1159/000130985. [DOI] [PubMed] [Google Scholar]
  13. Hatlen L., Attardi G. Proportion of HeLa cell genome complementary to transfer RNA and 5 s RNA. J Mol Biol. 1971 Mar 28;56(3):535–553. doi: 10.1016/0022-2836(71)90400-1. [DOI] [PubMed] [Google Scholar]
  14. Hovemann B., Sharp S., Yamada H., Söll D. Analysis of a drosophila tRNA gene cluster. Cell. 1980 Apr;19(4):889–895. doi: 10.1016/0092-8674(80)90080-x. [DOI] [PubMed] [Google Scholar]
  15. Kubli E. The structure and function of tRNA genes of higher eukaryotes. Experientia. 1981 Jan 15;37(1):1–9. doi: 10.1007/BF01965537. [DOI] [PubMed] [Google Scholar]
  16. LITTLEFIELD J. W. SELECTION OF HYBRIDS FROM MATINGS OF FIBROBLASTS IN VITRO AND THEIR PRESUMED RECOMBINANTS. Science. 1964 Aug 14;145(3633):709–710. doi: 10.1126/science.145.3633.709. [DOI] [PubMed] [Google Scholar]
  17. Müller F., Clarkson S. G. Nucleotide sequence of genes coding for tRNAPhe and tRNATyr from a repeating unit of X. laevis DNA. Cell. 1980 Feb;19(2):345–353. doi: 10.1016/0092-8674(80)90509-7. [DOI] [PubMed] [Google Scholar]
  18. Naylor S. L., Elliott R. W., Brown J. A., Shows T. B. Mapping of aminoacylase-1 and beta-galactosidase-A to homologous regions of human chromosome 3 and mouse chromosome 9 suggests location of additional genes. Am J Hum Genet. 1982 Mar;34(2):235–244. [PMC free article] [PubMed] [Google Scholar]
  19. Olson M. V., Hall B. D., Cameron J. R., Davis R. W. Cloning of the yeast tyrosine transfer RNA genes in bacteriophage lambda. J Mol Biol. 1979 Jan 25;127(3):285–295. doi: 10.1016/0022-2836(79)90330-9. [DOI] [PubMed] [Google Scholar]
  20. Owerbach D., Rutter W. J., Cooke N. E., Martial J. A., Shows T. B. The prolactin gene is located on chromosome 6 in humans. Science. 1981 May 15;212(4496):815–816. doi: 10.1126/science.7221563. [DOI] [PubMed] [Google Scholar]
  21. Owerbach D., Rutter W. J., Martial J. A., Baxter J. D., Shows T. B. Genes for growth hormone, chorionic somatommammotropin, and growth hormones-like gene on chromosome 17 in humans. Science. 1980 Jul 11;209(4453):289–292. doi: 10.1126/science.7384802. [DOI] [PubMed] [Google Scholar]
  22. Rigby P. W., Dieckmann M., Rhodes C., Berg P. Labeling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase I. J Mol Biol. 1977 Jun 15;113(1):237–251. doi: 10.1016/0022-2836(77)90052-3. [DOI] [PubMed] [Google Scholar]
  23. Roy K. L., Cooke H., Buckland R. Nucleotide sequence of a segment of human DNA containing the three tRNA genes. Nucleic Acids Res. 1982 Nov 25;10(22):7313–7322. doi: 10.1093/nar/10.22.7313. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Santos T., Zasloff M. Comparative analysis of human chromosomal segments bearing nonallelic dispersed tRNAimet genes. Cell. 1981 Mar;23(3):699–709. doi: 10.1016/0092-8674(81)90433-5. [DOI] [PubMed] [Google Scholar]
  25. Shows T. B., Brown J. A. Human X-Linked genes regionally mapped utilizing X-autosome translocations and somatic cell hybrids. Proc Natl Acad Sci U S A. 1975 Jun;72(6):2125–2129. doi: 10.1073/pnas.72.6.2125. [DOI] [PMC free article] [PubMed] [Google Scholar]
  26. Shows T. B. Genetics of human-mouse somatic cell hybrids: linkage of human genes for lactate dehydrogenase-A and esterase-A 4 . Proc Natl Acad Sci U S A. 1972 Feb;69(2):348–352. doi: 10.1073/pnas.69.2.348. [DOI] [PMC free article] [PubMed] [Google Scholar]
  27. Shows T. B., Sakaguchi A. Y., Naylor S. L. Mapping the human genome, cloned genes, DNA polymorphisms, and inherited disease. Adv Hum Genet. 1982;12:341–452. doi: 10.1007/978-1-4615-8315-8_5. [DOI] [PubMed] [Google Scholar]
  28. Southern E. M. Detection of specific sequences among DNA fragments separated by gel electrophoresis. J Mol Biol. 1975 Nov 5;98(3):503–517. doi: 10.1016/s0022-2836(75)80083-0. [DOI] [PubMed] [Google Scholar]
  29. Telford J. L., Kressmann A., Koski R. A., Grosschedl R., Müller F., Clarkson S. G., Birnstiel M. L. Delimitation of a promoter for RNA polymerase III by means of a functional test. Proc Natl Acad Sci U S A. 1979 Jun;76(6):2590–2594. doi: 10.1073/pnas.76.6.2590. [DOI] [PMC free article] [PubMed] [Google Scholar]
  30. Wahl G. M., Stern M., Stark G. R. Efficient transfer of large DNA fragments from agarose gels to diazobenzyloxymethyl-paper and rapid hybridization by using dextran sulfate. Proc Natl Acad Sci U S A. 1979 Aug;76(8):3683–3687. doi: 10.1073/pnas.76.8.3683. [DOI] [PMC free article] [PubMed] [Google Scholar]
  31. Wigler M., Sweet R., Sim G. K., Wold B., Pellicer A., Lacy E., Maniatis T., Silverstein S., Axel R. Transformation of mammalian cells with genes from procaryotes and eucaryotes. Cell. 1979 Apr;16(4):777–785. doi: 10.1016/0092-8674(79)90093-x. [DOI] [PubMed] [Google Scholar]
  32. Yen P. H., Sodja A., Cohen M., Jr, Conrad S. E., Wu M., Davidson N. Sequence arrangement of tRNA genes on a fragment of Drosophila melanogaster DNA cloned in E. coli. Cell. 1977 Aug;11(4):763–777. doi: 10.1016/0092-8674(77)90290-2. [DOI] [PubMed] [Google Scholar]
  33. Zasloff M., Santos T. Reiteration frequency mapping: analysis of repetitive sequence organization within cloned DNA fragments containing the human initiator methionine tRNA gene. Proc Natl Acad Sci U S A. 1980 Oct;77(10):5668–5672. doi: 10.1073/pnas.77.10.5668. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Proceedings of the National Academy of Sciences of the United States of America are provided here courtesy of National Academy of Sciences

RESOURCES