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. 1983 Aug;80(16):5061–5065. doi: 10.1073/pnas.80.16.5061

cDNA clone spanning the alpha-gamma subunit junction in the precursor of the murine fourth complement component (C4).

R T Ogata, D C Shreffler, D S Sepich, S P Lilly
PMCID: PMC384188  PMID: 6192448

Abstract

cDNA clones carrying parts of murine fourth complement component (C4, serum substance protein) mRNA sequences have been identified by differential hybridization to mRNA from a high C4-producing strain, B10.WR, and a congeneic low C4 strain, B10.BR, followed by hybrid-selected translation and DNA sequence analysis. One clone, pMLC4/w7-2, encodes an open amino acid reading frame that includes four tandem arginine residues immediately preceding a sequence 85% homologous with the NH2-terminal sequence of the human C4 gamma-chain. The amino acid composition of the predicted sequence upstream of the tandem arginines matches quite closely with the composition of a similar sized peptide at the COOH terminus of the human C4 alpha chain. The latter result raises questions regarding the nature and extent of plasma-mediated postsynthetic processing of the C4 alpha-chain COOH terminus. The results also demonstrate that strain differences in plasma C4 levels (low C4 vs. high C4) reflect differences in steady-state levels of liver C4 mRNA in these strains.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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