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. 1983 Aug;80(16):5117–5121. doi: 10.1073/pnas.80.16.5117

L-glutamic acid: a neurotransmitter candidate for cone photoreceptors in human and rat retinas.

C Brandon, D M Lam
PMCID: PMC384200  PMID: 6136039

Abstract

We have combined immunocytochemical localization of L-aspartate aminotransferase (L-aspartate:2-oxoglutarate aminotransferase, EC 2.6.1.1; glutamic-oxaloacetic transaminase) with autoradiographic localization of high-affinity uptake sites for L-glutamate or L-aspartate to identify the neurotransmitters of mammalian photoreceptors. In both human and rat retinas, high aspartate aminotransferase immunoreactivity is found in cones but not in rods; certain putative bipolar and amacrine cells are also heavily stained. In the human retina, and perhaps also in the rat retina, cones possess a high-affinity uptake mechanism for L-glutamate but not L-aspartate, whereas rods and Müller (glial) cells take up both L-glutamate and L-aspartate. Taken together, our results indicate that (i) L-glutamate is much more likely than L-aspartate to be the transmitter for human cones, and possibly for cones of other mammalian species as well, and (ii) major differences exist between mammalian cones and rods in the transport and metabolism or utilization of L-aspartate and L-glutamate.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Altschuler R. A., Mosinger J. L., Harmison G. G., Parakkal M. H., Wenthold R. J. Aspartate aminotransferase-like immunoreactivity as a marker for aspartate/glutamate in guinea pig photoreceptors. Nature. 1982 Aug 12;298(5875):657–659. doi: 10.1038/298657a0. [DOI] [PubMed] [Google Scholar]
  2. Altschuler R. A., Neises G. R., Harmison G. G., Wenthold R. J., Fex J. Immunocytochemical localization of aspartate aminotransferase immunoreactivity in cochlear nucleus of the guinea pig. Proc Natl Acad Sci U S A. 1981 Oct;78(10):6553–6557. doi: 10.1073/pnas.78.10.6553. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Balcar V. J., Johnston G. A. The structural specificity of the high affinity uptake of L-glutamate and L-aspartate by rat brain slices. J Neurochem. 1972 Nov;19(11):2657–2666. doi: 10.1111/j.1471-4159.1972.tb01325.x. [DOI] [PubMed] [Google Scholar]
  4. Bruun A., Ehinger B. Uptake of certain possible neurotransmitters into retinal neurons of some mammals. Exp Eye Res. 1974 Nov;19(5):435–447. doi: 10.1016/0014-4835(74)90052-9. [DOI] [PubMed] [Google Scholar]
  5. Cervetto L., MacNichol E. F., Jr Inactivation of horizontal cells in turtle retina by glutamate and aspartate. Science. 1972 Nov 17;178(4062):767–768. doi: 10.1126/science.178.4062.767. [DOI] [PubMed] [Google Scholar]
  6. Chin C. A., Lam D. M. The uptake and release of [3H]glycine in the goldfish retina. J Physiol. 1980 Nov;308:185–195. doi: 10.1113/jphysiol.1980.sp013467. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Dowling J. E., Ripps H. Adaptation in skate photoreceptors. J Gen Physiol. 1972 Dec;60(6):698–719. doi: 10.1085/jgp.60.6.698. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Ehinger B., Falck B. Autoradiography of some suspected neurotransmitter substances: GABA glycine, glutamic acid, histamine, dopamine, and L-dopa. Brain Res. 1971 Oct 8;33(1):157–172. doi: 10.1016/0006-8993(71)90314-3. [DOI] [PubMed] [Google Scholar]
  9. Ishida A. T., Fain G. L. D-aspartate potentiates the effects of L-glutamate on horizontal cells in goldfish retina. Proc Natl Acad Sci U S A. 1981 Sep;78(9):5890–5894. doi: 10.1073/pnas.78.9.5890. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Lam D. M., Ayoub G. S. Biochemical and biophysical studies of isolated horizontal cells from the teleost retina. Vision Res. 1983;23(4):433–444. doi: 10.1016/0042-6989(83)90090-1. [DOI] [PubMed] [Google Scholar]
  11. Lam D. M. Biosynthesis of gamma-aminobutyric acid by isolated axons of cone horizontal cells in the goldfish retina. Nature. 1975 Mar 27;254(5498):345–347. doi: 10.1038/254345a0. [DOI] [PubMed] [Google Scholar]
  12. Lam D. M., Fung S. C., Kong Y. C. Postnatal development of GABA-ergic neurons in the rabbit retina. J Comp Neurol. 1980 Sep 1;193(1):89–102. doi: 10.1002/cne.901930107. [DOI] [PubMed] [Google Scholar]
  13. Lam D. M., Hollyfield J. G. Localization of putative amino acid neurotransmitters in the human retina. Exp Eye Res. 1980 Dec;31(6):729–732. doi: 10.1016/s0014-4835(80)80059-5. [DOI] [PubMed] [Google Scholar]
  14. Lam D. M., Steinman L. The uptake of ( - 3 H) aminobutyric acid in the goldfish retina. Proc Natl Acad Sci U S A. 1971 Nov;68(11):2777–2781. doi: 10.1073/pnas.68.11.2777. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Lam D. M., Su Y. Y., Swain L., Marc R. E., Brandon C., Wu J. Y. Immunocytochemical localisation of L-glutamic acid decarboxylase in the goldfish retina. Nature. 1979 Apr 5;278(5704):565–567. doi: 10.1038/278565a0. [DOI] [PubMed] [Google Scholar]
  16. Lasater E. M., Dowling J. E. Carp horizontal cells in culture respond selectively to L-glutamate and its agonists. Proc Natl Acad Sci U S A. 1982 Feb;79(3):936–940. doi: 10.1073/pnas.79.3.936. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Marc R. E., Lam D. M. Glycinergic pathways in the goldfish retina. J Neurosci. 1981 Feb;1(2):152–165. doi: 10.1523/JNEUROSCI.01-02-00152.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Marc R. E., Lam D. M. Uptake of aspartic and glutamic acid by photoreceptors in goldfish retina. Proc Natl Acad Sci U S A. 1981 Nov;78(11):7185–7189. doi: 10.1073/pnas.78.11.7185. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Marc R. E., Stell W. K., Bok D., Lam D. M. GABA-ergic pathways in the goldfish retina. J Comp Neurol. 1978 Nov 15;182(2):221–244. doi: 10.1002/cne.901820204. [DOI] [PubMed] [Google Scholar]
  20. McLennan H. The autoradiographic localization of L-[3h]glutamate in rat brain tissue. Brain Res. 1976 Oct 8;115(1):139–144. doi: 10.1016/0006-8993(76)90828-3. [DOI] [PubMed] [Google Scholar]
  21. Murakami M., Otsu K., Otsuka T. Effects of chemicals on receptors and horizontal cells in the retina. J Physiol. 1972 Dec;227(3):899–913. doi: 10.1113/jphysiol.1972.sp010065. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Murakami M., Otsuka T., Shimazaki H. Effects of aspartate and glutamate on the bipolar cells in the carp retina. Vision Res. 1975 Mar;15(3):456–458. doi: 10.1016/0042-6989(75)90101-7. [DOI] [PubMed] [Google Scholar]
  23. Negishi K., Drujan B. D. Effects of some amino acids on horizontal cells in the fish retina. J Neurosci Res. 1979;4(5-6):351–363. doi: 10.1002/jnr.490040504. [DOI] [PubMed] [Google Scholar]
  24. Rowe J. S., Ruddock K. H. Depolarization of retinal horizontal cells by excitatory amino acid neurotransmitter agonists. Neurosci Lett. 1982 Jun 30;30(3):257–262. doi: 10.1016/0304-3940(82)90409-8. [DOI] [PubMed] [Google Scholar]
  25. White R. D., Neal M. J. The uptake of L-glutamate by the retina. Brain Res. 1976 Jul 23;111(1):79–93. doi: 10.1016/0006-8993(76)91050-7. [DOI] [PubMed] [Google Scholar]
  26. Wilkin G. P., Garthwaite J., Balázs R. Putative acidic amino acid transmitters in the cerebellum. II. Electron microscopic localization of transport sites. Brain Res. 1982 Jul 22;244(1):69–80. doi: 10.1016/0006-8993(82)90905-2. [DOI] [PubMed] [Google Scholar]
  27. Wu S. M., Dowling J. E. L-aspartate: evidence for a role in cone photoreceptor synaptic transmission in the carp retina. Proc Natl Acad Sci U S A. 1978 Oct;75(10):5205–5209. doi: 10.1073/pnas.75.10.5205. [DOI] [PMC free article] [PubMed] [Google Scholar]

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