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. 2013 Jul 6;61(8):1331–1346. doi: 10.1002/glia.22518

Figure 5.

Figure 5

Microglia depletion and addition of DKK2 partially rescue MDCK apoptosis. (A) Clodronate (100 µg/mL) effectively reduced the microglial markers csf1r and f4/80 in whole brain slices after 48 h of stimulation. Gene expression levels were normalized to the average of the respective housekeeping genes and expressed as fold changes (fc). Data represent mean ± SEM with n ≥ 9 of three individual experiments. (B1 and B2) Representative confocal images of the live (Calcein-AM = green) and dead (PI = red) staining of the epithelial MDCK cells at Position 1. Microglia depletion leads to a partial rescue of MDCK viability after clodronate treatment (scale bars represent 100 µm). (C) Image J quantification: Clodronate or DKK2 treatment resulted in a partial rescue of the glia-induced MDCK apoptosis at Position 1. Data represent mean ± SEM with n ≥ 9 of three individual experiments. [Color figure can be viewed in the online issue, which is available at wileyonlinelibrary.com.]