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. 1983 Sep;80(17):5217–5221. doi: 10.1073/pnas.80.17.5217

Methanophosphagen: Unique cyclic pyrophosphate isolated from Methanobacterium thermoautotrophicum

Sushila Kanodia 1, Mary Fedarko Roberts 1
PMCID: PMC384223  PMID: 16593359

Abstract

A unique cyclic pyrophosphate compound has been detected at 10-12 mM intracellular concentration in Methanobacterium thermoautotrophicum by in vivo31P NMR. This compound has been extracted from cells and purified by anion-exchange chromatography. Studies with 1H, 13C, and 31P NMR and fast-atom-bombardment mass spectrometry have identified it as 2,3-cyclopyrophosphoglycerate, an intramolecularly cyclized pyrophosphate of 2,3-diphosphoglycerate. Chemical degradation to 2,3-diphosphoglycerate and synthesis by dicyclohexylcarbodiimide coupling of 2,3-diphosphoglycerate are consistent with this identification. It is suggested that this compound serves as a primary phosphagen in methanogens.

Keywords: methanogens; 31P NMR; 2,3-cyclopyrophosphoglycerate; phosphagen

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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