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. 1983 Sep;80(18):5550–5553. doi: 10.1073/pnas.80.18.5550

Isolation of Z-DNA-containing plasmids.

R Thomae, S Beck, F M Pohl
PMCID: PMC384295  PMID: 6351062

Abstract

A purified, monoclonal antibody, specific for the left-handed Z-form of poly(dG-dC), was coupled covalently to Sephacryl S-1000 beads. Such an antibody column provides a convenient method to isolate and purify those plasmid DNAs that contain Z-DNA from a large excess of other DNAs, RNA, etc. From a library of Escherichia coli DNA, cloned into the vector plasmid pUC-8, several recombinant plasmids were isolated, which bind to this antibody. Thus, E. coli contains sequences, which in "natural" negatively supercoiled DNA, adopt a left-handed Z-DNA-like conformation.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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