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. 1983 Sep;80(18):5772–5775. doi: 10.1073/pnas.80.18.5772

Evidence for a proenkephalin-like precursor in amphibian brain.

D L Kilpatrick, R D Howells, H W Lahm, S Udenfriend
PMCID: PMC384341  PMID: 6412233

Abstract

The mammalian proenkephalin-derived peptides [Leu]enkephalin, [Met]enkephalin, and [Met]enkephalin-Arg6-Phe7 were identified in acid extracts of the brain of Bufo marinus by using reversed-phase HPLC and specific radioimmunoassays. [Met]Enkephalin was the predominant opioid peptide present (270 pmol/g). In contrast, the octapeptide [Met]enkephalin-Arg6-Gly7-Leu8, which is also derived from mammalian proenkephalin, was not detected. The ratio of free [Met]enkephalin to [Met]enkephalin-Arg6-Phe7 was found to be 3.5 to 1, which is similar to that observed in mammalian proenkephalin-containing tissues. Together these data (i) indicate that amphibian brain contains a proenkephalin related to the mammalian precursor and (ii) establish the existence of enkephalins and proenkephalin-derived enkephalin-containing peptides in a submammalian species.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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