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. Author manuscript; available in PMC: 2014 Aug 20.
Published in final edited form as: Biochemistry. 2013 Aug 2;52(33):10.1021/bi400378e. doi: 10.1021/bi400378e

Figure 3.

Figure 3

Trimer stability of the PCNA mutant proteins. (A) Analysis of the PCNA proteins by native gel electrophoresis. Solutions containing the wild-type or mutant PCNA proteins (0.1 to 1.0 mg/ml) were run on a non-denaturing polyacrylamide gradient gel (4 to 20%) and Coomassie stained. The position of the PCNA trimer is indicated. (B) Analysis of the PCNA proteins by size exclusion chromatography. The elution profile of a size exclusion chromatography column in which a solution of the wild-type PCNA protein, the C22Y mutant PCNA proteins, and the C81R mutant PCNA protein (10 mg/ml) were run are shown.