Fig. 3.

Metformin inhibits growth of PCa cells. (A–C) Myc-CaP, C4-2b and RWPE-1 cells were 2mM metformin for up to 72 h or (D–F) at indicated concentrations for 48h or left untreated (control, PBS vehicle). Following treatment, cell density was measured using the Alamar-Blue assay as described in Materials and methods. Each bar represents the mean ± SD of three independent samples. *P < 0.05, **P < 0.01 when compared with vehicle. Representative photos of each cell type treated with metformin or vehicle are shown.