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. Author manuscript; available in PMC: 2013 Dec 2.
Published in final edited form as: Neuron. 2013 Feb 20;77(4):10.1016/j.neuron.2012.12.031. doi: 10.1016/j.neuron.2012.12.031

Figure 4. PLX-1 functions throughout development.

Figure 4

(A and B) Synaptic tiling at early L1 stage of wild type (A) and plx-1 mutants (B). A tiling defect was observed at this stage in the plx-1 mutants (yellow arrow). Scale bar, 10μm. (C) Quantification of DA8/DA9 overlap at L4 stage. Two independent transgenic lines and their siblings without the transgenes were quantified. Heat shock induction of plx-1 gene expression from the hsp promoter at the embryonic (red bars) and larval (orange bars) stages significantly rescued the plx-1 mutant phenotype while there was no significant difference between transgenic animals and non-transgenic siblings without heat shock (white bars). Error bars; standard error of mean. Triple asterisks: p<0.001, n.s.: not significant (ANOVA/Tukey-HSD). (D) Schematic representation of worm developmental time course and the timing of heat shock and imaging.