BIOCHEMISTRY. For the article “Redox regulation of surface protein thiols: Identification of integrin α-4 as a molecular target by using redox proteomics,” by Teresa Laragione, Valentina Bonetto, Filippo Casoni, Tania Massignan, Giancarlo Bianchi, Elisabetta Gianazza, and Pietro Ghezzi, which appeared in issue 25, December 9, 2003, of Proc. Natl. Acad. Sci. USA (100, 14737-14741; first published December 1, 2003; 10.1073/pnas.2434516100), the authors note that the x-axis label for the NAC concentration in Fig. 1B should be micromolar. In addition, the indications for the black and white bars were switched in the legend for Fig. 6. The legend should have read: “No antibody, black bars; anti-VLA-4 (10 ng/ml), gray bars; and anti-VLA-4 (10 μg/ml), white bars.” The corrected figures and their legends appear below.
Fig. 1.
Time course (A) and dose response (B) of NAC-induced increase of surface SH expression. PBMCs (106 cells per ml) were cultured with 5 mM NAC for the indicated time (A), after which surface thiols were quantified with DTNB. In the experiment shown in B, cells were incubated for 2 h with the indicated concentration of NAC. Data are the mean of duplicate experiments.
Fig. 6.
Quantitation of the effect of NAC on cell adhesion. Experiments were performed exactly as in Fig. 5. Cells were solubilized and eosin Y and absorbance was quantitated at 540 nm. No antibody, black bars; anti-VLA-4 (10 ng/ml), gray bars; and anti-VLA-4 (10 μg/ml), white bars. Data are means ± SE (n = 3). *, P < 0.01 vs. control.